Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 11 to 14 amino acid residues in defined sequence
Reexamination Certificate
2006-09-05
2006-09-05
Russel, Jeffrey Edwin (Department: 1654)
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
11 to 14 amino acid residues in defined sequence
C435S005000, C435S091500, C436S086000, C502S007000, C530S810000
Reexamination Certificate
active
07101966
ABSTRACT:
Autographa californica nucleopolyhedrovirus (AcNPV) is a baculovirus widely employed as a vector for the expression of foreign genes and pest control. Although baculoviruses efficiently replicate in the nuclei of arthropod cells, the dynamics and mechanism of DNA replication within the infected cell are still poorly understood. Thus, to study such viral DNA replication, the availability of peptide ligands specific for DNA-binding protein (DBP) is needed. This work resulted in the selection of peptide ligands specifically binding to DBP for AcNPV from the FliTrx™ random peptide display library, which entails the amplification, cloning of the DBP gene from AcNPV, the construction of the expression plasinid for DBP, and the expression and purification of the recombinant His.Tag AcNPV DBP which was used as a target molecule for the selection of the peptide ligands. The affinity of peptide ligands was measured by ELISA procedures. The novelty and advantage of this invention is the monospecificity quality of the peptide ligands specific for AcNPV DBP which is valuable for diverse applications in basic as well as applied research.
REFERENCES:
patent: 6037135 (2000-03-01), Kubo et al.
patent: 6974791 (2005-12-01), Wong et al.
Laufs et al. Autographa californica Nuclear Polyhedrovirus Virus p143 Gene Product Is a DNA-Binding Protein. Virology. 1997, vol. 228, pp. 98-106.
Russel Jeffrey Edwin
Vista Biologicals Corporation
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