Selection of intracellular immunoglobulins

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C435S007100, C435S007200, C435S069100, C435S069700, C435S328000, C435S332000, C436S512000, C436S513000, C436S517000, C436S056000, C436S164000, C530S386000, C530S387100, C530S387300, C530S387700, C530S388100, C530S388210

Reexamination Certificate

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07569390

ABSTRACT:
A general immunoglobulin-target assay system is provided, in which a positive outcome (the generation of a signal) depends only on the intracellular interaction of immunoglobulin with target. This can be accomplished for many immunoglobulins expressed in yeast and/or in mammalian cells and allows the selection of immunoglobulins which are capable of functioning in an intracellular environment.

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Visintin, Michela et al. “Selection of antibodies for intracellular function using a two-hybrid in vivo system.” Proceedings of the National Academy of Sciences of the United States, vol. 96, No. 21, pp. 11723-11728. Oct. 12, 1999.
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