Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1997-06-23
1999-03-02
Chambers, Jasemine C.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 6, 4352523, 43525411, C12N 1509, C12N 115, C12N 121, C12Q 168
Patent
active
058769889
ABSTRACT:
The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
REFERENCES:
patent: 5302527 (1994-04-01), Birkett et al.
patent: 5422267 (1995-06-01), Yocum et al.
Alani et al. (1987) "A method for gene disruption that allows repeated use of URA3 selection . . . " Genetics 116: 541-545.
Scherer et al. (1979) "Replacement of chromosome segments with altered DNA sequences constructed in vitro" Proc. Natl. Acad. Sci. USA 76:4951-4955.
Boeke et al. (1984) "A positve selection for mutants lacking orotidine 5' phosphate decarboxylase activity in yeast . . . " Mol Gen Genet 197:345-346.
Debets et al. (1990) Genetic analysis of amdS transformants of Aspergillus niger and their use in chromosome mapping. Mol. Gen. Genet. 222:284-290.
Casey, G. P. et al., "A Convenient Dominant Selection Marker for Gene Transfer in Industrial Strains of Saccharomyces Yeast: SMRJ Encoded Resistance to the Herbicide Sulfometuron Methyl" J. Inst. Brew. (1988) 94:93-97.
Chang, T.-H. et al., "Identification of a putative amidase gene in yeast Saccharomyces cerevisiae," Nucleic Acids Research (1990) 18(23):7180.
Gomi, K. et al., "Cloning and molecular characterization of the acetamidase-encoding gene (amdS) from Aspergillus oryzae," Gene (1991) 108:91-98.
Kelly, J. M. et al., "Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans" EMBO J. (1985) 4:475-479.
Mahenthiralingam, E. et al., "Cloning and sequencing of the gene which encodes the highly inducible acetamidase of Mycobacterium smegmatis," J Gen Microbiol (1993) 139(3):575-83.
Selten Gerardus Cornelius Maria
Swinkels Bart Willem
Van Gorcom Robertus Franciscus Maria
Chambers Jasemine C.
Gist-brocades BV
Priebe Scott D.
LandOfFree
Selection marker gene free recombinant strains: method for obtai does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Selection marker gene free recombinant strains: method for obtai, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Selection marker gene free recombinant strains: method for obtai will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-421742