Selection marker gene free recombinant strains: method for obtai

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 6, 4352523, 43525411, C12N 1509, C12N 115, C12N 121, C12Q 168

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active

058769889

ABSTRACT:
The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.

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Casey, G. P. et al., "A Convenient Dominant Selection Marker for Gene Transfer in Industrial Strains of Saccharomyces Yeast: SMRJ Encoded Resistance to the Herbicide Sulfometuron Methyl" J. Inst. Brew. (1988) 94:93-97.
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