Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1997-12-08
2000-04-18
Brusca, John S.
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
435479, 435483, 435485, 435488, C12N 1587, C12N 1574, C12N 1575
Patent
active
060514310
ABSTRACT:
The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
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Selten Gerardus Cornelius Maria
Swinkels Bart Willem
Van Gorcom Robertus Franciscus Maria
Brusca John S.
DSM N.V.
Sandals William
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