Selection marker gene free recombinant strains

Details Selection marker gene free recombinant strains Selection marker gene free recombinant strains

2005-10-18

2005-10-18

Guzo, David (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S243000, C435S252300, C435S252330

Reexamination Certificate

active

06955909

ABSTRACT:
The present invention discloses a selection marker free system which can be used to introduce genetic modifications to in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.

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