Selecting, culturing and creating lineage committed...

Details Selecting, culturing and creating lineage committed... Selecting, culturing and creating lineage committed...

2006-04-20

2011-12-06

Gamett, Daniel C (Department: 1647)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C530S388700

Reexamination Certificate

active

08071367

ABSTRACT:
The present invention provides a method for selecting hematopoietic stem cells (HSCs) comprising providing an agent which binds to α9β1 integrin on the cell surface to a population of cells including HSCs and separating HSCs by virtue of the binding agent. The invention also provides a method of culturing a population of HSCs in the presence of an agent which binds to α9β1, wherein the agent inhibits differentiation of the HSCs. The invention also provides a method of producing a population of lineage committed cells comprising culturing HSCs in the presence of an agent which inhibits or prevents binding to α9β1.

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JP 2003-189843, published Jul. 8, 2003, English translation accessed Feb. 25, 2010.
Iwata et al., “Human marrow stromal cells activate monocytes to secrete osteopontin, which down-regulates Notch1 gene expression in CD34+ cells” Blood, 103(12):4496-4502 (Jun. 16, 2004).
Nilsson, et al. Osteopontin, a key component of the hematopoietic stem cell niche and regulator of primitive hematopoietic progenitor cells. 2005, Blood, vol. 106, No. 4, pp. 1232-1239.
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