Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1993-10-28
1995-12-12
Fox, David T.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 691, 4353201, 4351723, 4352523, A01H 104, C12N 514, C12N 1500
Patent
active
054749298
ABSTRACT:
Adenosine deaminase gene (ADA gene) is used as a selectable/reporter gene for plant cells and plants. When introduced into plant cells, the ADA gene makes the cells resistant to inhibition by 2-deoxyadenosine or an analog, so transformed cells can be separated from non-transformed cells by culturing a cell mixture in a medium containing 2-deoxyadenosine or an analog in amounts that inhibit normal plant cells. A single vector may be constructed containing an ADA gene from any source (e.g. a mouse ADA gene) and an additional gene-of-interest to be introduced into plant cells. The vector may then be introduced into the cells by such a route as Agrobacterium infection or micro-projectile bombardment. The presence of the ADA gene in successfully transformed plant cells may be confirmed by cultivating the cells in a medium containing 2-deoxyadenosine or an analog in the presence of a material that changes color in the presence of ammonia. Observation of a color change confirms the presence of the gene. DNA constructs containing both ADA genes and foreign genes-of-interest may be constructed, e.g. plasmid pRD360-ADA (ATCC 69459).
REFERENCES:
patent: 4822736 (1989-04-01), Kellems et al.
patent: 5073675 (1989-05-01), Jones et al.
patent: 5166059 (1992-11-01), Pastan et al.
Kellems et al; "Adenosine Deaminase: A Dominant Amplifiable Genetic Marker For Use in Mammalian Cells" Gen. and Mol. Biol. of Ind. Micro. 1989 pp. 215-225.
Yeung et al: "Identification of Functional Murine Adenosine . . . " The Journal of Biological Chemistry--1985 vol. 260, No. 18 pp. 10299-10307.
van Beusechem et al; Proc. Natl. Acad. Sci. USA, vol. 89, pp. 7640-7644 Aug. 92 "Long-term expression of human adenosine deaminase . . . ".
Shen et al; Gene, 98 (1991) 283-287 "Construction and expression of an adenosine deaminase::lacZ fusion gene".
Yabuki et al; Biochimica et Biophysica acta, 1073 (1991) 474-480 "Catabolism of adenine nucleotides in suspension-cultured plant cell".
Butters et al; Physiological Plant Pathology (1985) 27 pp. 65-74 "Purine Metabolism in barley powdery mildew and its host".
Kaufman et al (1986) Proc Natl Acad Sci, USA 83: 3136-3140.
Fox David T.
McElwain Elizabeth F.
National Research Council of Canada
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