Selectable marker for development of vectors and transformation

Details Selectable marker for development of vectors and transformation Selectable marker for development of vectors and transformation

1990-09-19

2000-04-11

Fox, David T.

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

435 691, 435 697, 435194, 4353201, 435418, 435419, 435469, 800288, 800294, 800300, 536 234, 536 237, C12N 1554, C12N 504, C12N 1582, C12N 1584

Patent

active

060487305

ABSTRACT:
The present invention discloses functional and selectable micro-Ti plasmids. The hygromycin phosphotransferase (aphIV) gene from Escherichia coli was inserted between the 5' promoter and associated amino terminal region-encoding sequence of an octopine synthetase gene and the 3' terminator signal sequence of a nopaline synthetase gene. These constructs were assembled between T-DNA border fragments in a broad-host-range vector and used to create antibiotic-resistant plant cells.

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