Selectable marker for development of vectors and transformation

Details Selectable marker for development of vectors and transformation Selectable marker for development of vectors and transformation

1995-06-07

1997-09-16

Fox, David T.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

800250, 800255, 536 232, 536 234, 536 237, 435 691, 435 697, 435 701, 4351723, 435194, 4352522, 4353201, 435418, A01H 500, A01H 510, C12N 1531, C12N 1584, C12N 504

Patent

active

056682980

ABSTRACT:
The present invention discloses functional and selectable micro-Ti plasmids. The hygromycin phosphotransferase (aphIV) gene from Escherichia coli was inserted between the 5' promoter and associated amino terminal region-encoding sequence of an octopine synthetase gene and the 3' terminator signal sequence of a nopaline synthetase gene. These constructs were assembled between T-DNA border fragments in a broad-host-range vector and used to create antibiotic-resistant plant cells.

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