Segment of glycosylation-deficient HGFalpha-chain

Details Segment of glycosylation-deficient HGFalpha-chain Segment of glycosylation-deficient HGFalpha-chain

2004-08-26

2010-10-05

Kolker, Daniel E. (Department: 1649)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Hormone or other secreted growth regulatory factor,...

C530S350000, C435S440000

Reexamination Certificate

active

07807177

ABSTRACT:
The present invention provides a segment of glycosylation-deficient HGF having mutation(s) introduced into an amino acid sequence so as to prevent glycosylation at at least one glycosylation site of a hepatocyte growth factor (HGF), and a method of producing the same. The segment of glycosylation-deficient HGF of the present invention has the same activity as that of a segment of glycosylated HGF, therefore, it is useful as an alternate for a segment of glycosylated HGF.

REFERENCES:
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R. Hofmann et al., “Scatter factor is a glycoprotein but glycosylation is not required for its activity”, Biochimica et Biophysica Acta, vol. 1120, No. 3, pp. 343-350, 1992.
N. Shimizu et al., “Hepatocyte Growth Factor is Linked by O-Glycosylated Oligosaccharide on the α Chain”, Biochemical and Biophysical Research Communications, vol. 189, No. 3, pp. 1329-1335, Dec. 30, 1992.
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