Seed-specific promoter from the rice glutelin GluB-4 gene...

Details Seed-specific promoter from the rice glutelin GluB-4 gene... Seed-specific promoter from the rice glutelin GluB-4 gene...

2006-05-01

2009-11-17

Worley, Cathy Kingdon (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide contains a tissue, organ, or cell...

C435S320100, C435S419000, C800S298000, C536S024100

Reexamination Certificate

active

07619135

ABSTRACT:
An objective of the present invention is to provide promoters having seed-specific promoter activity, and methods of expressing foreign proteins in seeds. In particular, a seed specific promoter from the rice glutelin GluB-4 gene is disclosed. The present inventors isolated the promoters of a number of genes that are expressed in rice seeds, constructed binary vectors in which each promoter is inserted upstream of the GUS reporter gene, and transformed rice using theAgrobacteriummethod. The inventors then used GUS expression level as an index to examine the site of expression, the expression pattern during seed maturation, and the level of expression in seeds for each promoter. They thus discovered promoters with activity specific to a particular site in seeds, and with higher activity than constitutive promoters and known seed-specific promoters.

REFERENCES:
patent: 5294593 (1994-03-01), Khan
patent: 7332318 (2008-02-01), Shimizu et al.
patent: 7402418 (2008-07-01), Osumi et al.
patent: 2002/0081590 (2002-06-01), Penn et al.
patent: 2003/0135884 (2003-07-01), Yu et al.
patent: 2003/0159182 (2003-08-01), Tackaberry et al.
patent: 2005/0066387 (2005-03-01), Yu et al.
patent: 2005/0223438 (2005-10-01), Otsuki et al.
patent: 2006/0191044 (2006-08-01), Takaiwa et al.
patent: 2002-58492 (2002-02-01), None
patent: 2002-209462 (2002-07-01), None
patent: 2002-315582 (2002-10-01), None
patent: 2003-33190 (2003-04-01), None
patent: 2004-159638 (2004-06-01), None
patent: WO 99/16890 (1999-04-01), None
patent: WO 03/000905 (2003-01-01), None
patent: WO 03/000905 (2003-01-01), None
patent: WO 03/079769 (2003-10-01), None
patent: WO 2004/094637 (2004-04-01), None
patent: WO 2004/056993 (2004-08-01), None
Donald et al. Mutation of either G box or I box sequences profoundly affects expression from theArabidopsis rbcS-1A promoter. (1990) The EMBO Journal, vol. 9, pp. 1717-1726.
Benfey et al. The cauliflower mosaic virus 35S promoter: combinatorial regulation of transcription in plants. (1990) Science, vol. 250, pp. 959-966.
Kim et al. A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity. (1994) PMB, vol. 24, pp. 105-117.
Jiang et al. New rice glutelin gene GluB-4 (2002) GenBank Accession AF537221, pp. 1-3.
Sasaki et al.Oryza sativanipponbare (GA3) genomic DNA, chromosome 2, BAC clone:OJ1005—D05. (2002) GenBank Accession AP004128, pp. 1-26.
Lazo et al. A DNA transformation competentArabidopsisgenomic library inAgrobacterium. (1991) Biotechnology; vol. 9; pp. 963-967.
Su et al.Oryza sativa10 kDa prolamin gene, complete cds. (2001) GenBank Accession AF294580; pp. 1-2.
Croissant-Sych, et al. “Identification of Positive and Negative Regulatorycis-Elements of the Rice GlutelinGt3Promoter,”Plant Science, vol. 116, pp. 27-35, 1996.
Daniell, et al. “Medical Molecular Farming: Production of Antibodies, Biopharmaceuticals and Edible Vaccines in Plants,”Trends in Plant Science, vol. 6, No. 5, pp. 219-226, May 2001.
Delaney, “Choice of Crop Species and Development of Transgenic Product Lines,” inPlants as Factories for Protein Production, pp. 139-158 E. Hood and J. Howard, editors, Kluwer Academic Publishers, 2002.
Fischer, et al. “Molecular Farming of Pharmaceutical Proteins,”Transgenic Research, vol. 9, pp. 279-299, 2000.
Giddings et al. “Transgenic Plants as Factories for Biopharmaceuticals,”Nature Biotechnology, vol. 18, pp. 1151-1155, Nov. 2000.
Howard, et al., “Introduction to Molecular Farming,”Plants as Factories for Protein Production, pp. vii-x, E. Hood and J. Howard, editors, Kluwer Academic Publishers, 2002.
Takaiwa, et al. “Characterization of Commoncis-Regulatory Elements Responsible for the Endosperm-Specific Expression of Members of the Rice Glutelin Multigene Family,”Plant Molecular Biology, vol. 30, pp. 1207-1221, 1996.
Takaiwa, et al. “Analysis of the 5″ Flanking Region Responsible for the Endosperm-Specific Expression of a Rice Glutelin Chimeric Gene in Transgenic Tobacco,”Plant Molecular Biology, vol. 16, pp. 49-58, 1991.
Walmsley, et al. “Plants for Delivery of Edible Vaccines,”Plant Biotechnology, vol. 11, No. 2, pp. 126-129, 2000.
Wu, et al. “Quantitative Nature of the Prolamin-box, ACGT and AACA Motifs in a Rice Glutelin Gene Promoter: Minimalcis-Element Requirements for Endosperm-Specific Gene Expression,”The Plant Journal, vol. 23, No. 3, pp. 415-421, 2000.
Wu, et al. “The GCN4 Motif in a Rice Glutelin Gene is Essential for Endosperm-Specific Gene Expression and is Activated by Opaque-2 in Transgenic Rice Plants,”The Plant Journal, vol. 14, No. 6, pp. 673-683, 1998.
Yoshihara et al. “A 45-bp proximal Region Containing AACA and GCN4 Motif is Sufficient to Confer Endosperm-Specific Expression of the Rice Storage Protein Glutelin Gene,GluA-3,” FEBS Letters, vol. 383, pp. 213-218, 1996.
Zhao et al. “Tissue-Specific Expression and Temporal Regulation of the Rice Glutelin Gt3 Gene Are Conferred by at Least Two Spatially Separatedcis-Regulatory Elements,”Plant Molecular Biology, vol. 25, pp. 429-436, 1994.
Zheng, et al. “5′ Distal and Proximalcis-Acting Regulator Elements are Required for Developmental Control of a Rice Seed Storage ProteinGlutelinGene,”The Plant Journal, vol. 4, No. 2, pp. 357-366, 1993.
Kikuchi, et al. Molecular Characterization of a Gene for Alanine Aminotransferase from Rice (Oryza sativa)Plant Molecular Biology, vol. 39, pp. 149-159, 1999.
Patel, et al. “Transgenic Barley Expressing a Fungal Xylanase Gene in the Endosperm of the Developing Grains,”Molecular Breeding, vol. 6, pp. 113-123, 2000.
Qu, et al. “Evaluation of Tissue Specificity and Expression Strength of Rice Seed Component Gene Promoters in Transgenic Rice,”Plant Biotechnology Journal, vol. 2, pp. 113-125, 2004.
Wu, et al. “Promoters of Rice Seed Storage Protein Genes Direct Endosperm-Specific Gene Expression in Transgenic Rice,”Plant Cell Physiology, vol. 39, No. 8, pp. 885-889, 1998.
Kim, et al. A 20 Nucleotide Upstream Element is Essential for the Nopaline Synthase (nos) Promoter Activity. (1994) PMB, vol. 24, pp. 105-117.
Fumio.O. sativaBluB-1 Gene for Glutelin. (1991) GenBank Accession #X54314.
Su, et al.Oryza sativa10 kDa Prolamin Gene, complete cds. (2001) GenBank Accession #AF294580.
Walmsley, et al. “Plants for Delivery of Edible Vaccines,”Current Opinion in Biotechnology, vol. 11, No. 2, pp. 126-129, 2000.
Katsube, et al. “Accumulation of Soybean Glycinin and Its Assembly with the Glutelins in Rice,”Plant Physiology, vol. 120, pp. 1063-1073, Aug. 1999.
Takaiwa, et al. “Sequence of Three Members and Expression of a New Major Subfamily of Glutelin Genes from Rice,”Plant Molecular Biology, vol. 17, pp. 875-885, 1991.
Takaiwa, et al. “Genomic DNA Sequences of Two New Genes for New Storage Protein Glutelin in Rice,”Japanese Journal of Genetics, vol. 66, pp. 161-171, 1991.
Washida, et al. “Identification ofcis-Regulatory Elements Required for Endosperm Expression of the Rice Storage Protein Glutelin GeneGluB-1,” Plant Molecular Biology, vol. 40, pp. 1-12, 1999.
Feng, et al. “Nucleotide Sequence of a Cloned Rice Genomic DNA Fragment that Encodes a 10 kDa Prolamin Polypeptide,” Nucleic Acids Research, vol. 18, p. 683, 1990.
Feng, et al. GenBank Accession X17074; pp. 1-2, 1993.
Stalberg, et al. “Deletion Analysis of a 2S Seed Storage protein Promoter ofBrassica napusin Transgenic Tobacco,”Plant Molecular Biology, vol. 23, pp. 671-683, 1993.
Chen, et al. “Oryza sativasubsp. japonic putative ADP-Glucose Phrophosphorylase Subunit SH2 . . . ” GenBank Accession AF101045, pp. 1-12.
Russell, et al. “Tissue-Specific Expression in Transgenic Maize of Four Endosperm Promoters from Maize and rice,”Transgenic Research, vol. 6, pp. 157-168, 1997.
Sun, et al. “Rice Embryo Globulins: Amino-

Affiliated with

Also associated with

Rating

Seed-specific promoter from the rice glutelin GluB-4 gene... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Seed-specific promoter from the rice glutelin GluB-4 gene..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Seed-specific promoter from the rice glutelin GluB-4 gene... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4117342