Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2007-03-20
2007-03-20
Kubelik, Anne (Department: 1638)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C800S287000, C435S320100, C435S419000, C536S024100
Reexamination Certificate
active
10978798
ABSTRACT:
An objective of the present invention is to provide promoters having seed-specific promoter activity, and methods of expressing foreign proteins in seeds. The present inventors isolated the promoters of a number of genes that are expressed in rice seeds, constructed binary vectors in which each promoter is inserted upstream of the GUS reporter gene, and transformed rice using theAgrobacteriummethod. In particular a seed-specific promoter from the rice glutelin GluB-1 gene is disclosed. GUS expression level was used as an index to examine the site of expression, the expression pattern during seed maturation, and the level of expression in seeds for each promoter. Promoters with activity specific to a particular site in seeds, and with higher activity than constitutive promoters and known seed-specific promoters were discovered.
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Qu Leqing
Takaiwa Fumio
Kingdon Worley Cathy
Knobbe Martens Olson & Bear LLP
Kubelik Anne
National Institute of Agrobiological Sciences
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