Seed-preferred promoter from Sorghum kafirin gene

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...

Reexamination Certificate

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C800S278000, C800S295000, C800S298000, C536S024100, C435S468000

Reexamination Certificate

active

07964770

ABSTRACT:
The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the sorghum δ-kafirin coding region. The sequences drive expression preferentially to endosperm tissue. A method for expressing a nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

REFERENCES:
patent: 2004/0088754 (2004-05-01), Cho et al.
Stalberg et al. Deletion anaylsis of a 2S seed storage protein promoter of Brassica napus in transgenic tobacco. (1993) PMB; vol. 23; pp. 671-683.
Bedell et al. 104—419—10941692—114—32295—080 Sorghum methylation-filtered library bicolor genomic clons 10941692, genomic survey sequence. (2004) GenBank Accession CL194520.1; p. 1.

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