Seed coat-specific cryptic promoter in tobacco

Details Seed coat-specific cryptic promoter in tobacco Seed coat-specific cryptic promoter in tobacco

1995-05-15

1998-10-20

McElwain, Elizabeth F.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

536 241, 435 691, 4351723, 4353201, 435418, 435419, A01H 500, C12N 1582

Patent

active

058248633

ABSTRACT:
T-DNA tagging with a promoterless .beta.-glucuronidase (GUS) gene generated a transgenic Nicotiana tabacum plant that expressed GUS activity only in developing seed coats. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promotor is useful in controlling the expression of genes to the developing seed coat in plant seeds.

REFERENCES:
T-DNA tagging of a seed coat-specific cryptic promoter in tobacco. The Pl Journal, (1994) 6(4), 567-577.
Detection of gene regulatory signals in plant revealed by T-DNA-mediated fusions. Plant Molecular Biology, (1991) 17:837-851.
Programs and Abstracts, Third International Congress of Plant Molecular Biology. Molecular Biology of Plant Growth and Development. 6-11 Oct. 1991, Tucson, Arizona, U.S.A.
T-DNA tagging of promoter-like regions. Poster Abstract Form. P. Fobert et al.
Kim et al. (1994) Plant Mol Biol 24: 105-117.

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