Chemistry: molecular biology and microbiology – Vector – per se
Patent
1987-05-01
1991-08-06
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Vector, per se
435 691, 435 698, 435 91, 4351721, 43525231, 43525235, 4352525, C12N 1500, C12N 120, C12P 2106, C12P 1934
Patent
active
050377604
ABSTRACT:
Novel vectors are provided for identifying secretory signal sequences from DNA fragments of unicellular microorganisms. The plasmids comprise a multiple cloning site with restriction sites in reading frame with a structural gene which permits rapid screening of the screted expression product. Optionally, the vectors may include a promoter region upstream from the multiple cloning site. The invention is exemplified with Bacillus. Specific secretory signal sequence have been isolated with those vectors, allowing for efficient secretion into the supernatant, and not just to the periplasmic space to provide proteins in economically high yields. Secretory sequences are provided superior to other previously known sequences.
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Pouwels et al., Cloning Vectors, A Laboratory Manual, Elsevier Publ. (1985).
Maniatis, T. et al. Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, CSH, NY (1982).
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Kazutaka, et al., Chemical Abstracts, vol. 100 Abstract No. 80657r (1984).
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Bron Sierd
Peeters Ben P. H.
Smith Hilde E.
Van Ee Jan H.
Venema Gerard
Ellis Joan
Gist-Brocades NV
Rae-Venter Barabara
Rowland Bertram I.
Schwartz Richard A.
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