Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1990-12-13
1993-05-18
Ellis, Joan
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 91, 4351723, 43525231, 43525233, 4353201, 536 237, 530300, 935 6, 935 29, 935 41, 935 48, 935 56, 935 60, 935 73, 935 74, 935 82, C12P 2102, C12P 1934, C12N 1500, C12N 121, C07H 1512, A61K 3702
Patent
active
052120708
ABSTRACT:
Novel vectors are provided for identifying secretory signal sequences from DNA fragments of unicellular microorganisms. The plasmids comprise a multiple cloning site with restriction sites in reading frame with a structural gene which permits rapid screening of the secreted expression product. Optionally, the vectors may include a promoter region upstream from the multiple cloning site. The invention is exemplified with Bacillus. Specific secretory signal sequences have been isolated with those vectors, allowing for efficient secretion into the supernatant, and not just to the periplasmic space to provide proteins in economically high yields. Secretory sequences are provided superior to other previously known sequences.
REFERENCES:
patent: 4663280 (1987-05-01), Sloma et al.
patent: 4711843 (1987-12-01), Chang et al.
patent: 4757013 (1988-07-01), Inouye et al.
Palva et al. Gene vol. 22 pp. 229-235 (1983).
Vieira et al. Gene vol. 19 pp. 259-268 (1982).
Pouwels et al Cloning Vectors, A Laboratory Manual Elsevier (1985).
Yary et al.--Nucl. Acids Res. vol. 11 pp. 237-249 (1983).
Ohmura et al J. Biochem. vol. 95 pp. 87-93 (1984).
Deute et al Nucl. Acids Res. vol. 11 pp. 1645-1655 (1983).
Bron Sierd
Peeters Ben P. H.
Smith Hilde E.
Van Ee Jan H.
Venema Gerard
Ellis Joan
Gist-Brocades
Rae-Venter Barbara
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