Secretory gene trap

Details Secretory gene trap Secretory gene trap

1995-06-30

1998-08-04

Crouch, Deborah

Multicellular living organisms and unmodified parts thereof and

Nonhuman animal

800DIG1, 435 911, 4351723, 4353201, 435325, C12N 500, C12N 1500, C12P 1934

Patent

active

057896534

ABSTRACT:
The invention relates to secretory gene trap vectors and methods of using such vectors to isolate extracellular proteins and to make cells and organisms with mutant secretory genes. The vectors encode a type II transmembrane domain and a secretory lumen -sensitive indicator marker and optionally, a selectable marker and an exon-splice acceptor site. The gene isolation methods involve stably introducing the secretory trap vectors into an endogenous gene whereby the expression of the resultant fusion protein provides a differential expression of the indicator marker depending on whether the endogenous gene provides an N-terminal signal sequence.

REFERENCES:
Molecullar Cloning : A Laboratory Manual, 2nd ed, Sambrook et al, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989, 1.13,1.85-1.86.

Affiliated with

Also associated with

Rating

Secretory gene trap does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Secretory gene trap, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Secretory gene trap will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1179041