Secretory expression in eukaryotes

Details Secretory expression in eukaryotes Secretory expression in eukaryotes

1987-08-03

1989-09-26

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

4351723, 435256, 435320, 935 37, 935 48, C12P 2102, C12N 1500, C12N 112, C12N 100

Patent

active

048700088

ABSTRACT:
Methods and compositions are provided for producing polypeptide sequences in high yield by employing DNA constructs, wherein the DNA sequence encoding from the polypeptide of interest in preceded by a leader sequence and processing sequence for secreting and processing said polypeptide. In this manner, the mature polypeptide of interest may be isolated from the nutrient medium substantially free of major amounts of other proteins and cellular debris.
The yeast strain S. cerevisiae AB103 (pYEGF8) was deposited on Jan. 5 , 1983, at the A.T.C.C. and given accession No. 20658.
The plasmid pY.alpha.EGF23 (pAB114-pCl/1) was deposited at the A.T.C.C. on Aug. 12, 1983, and given Accession No. 40079.

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Hishinuma et al., in Molecular Biology of Yeast: Abstracts of Papers Presented at the 1985 Meeting, p. 309 (Cold Spring Harbor Laboratory, 1985).
Julius et al. (1983), Cell, 32:839-852.
Brake et al. (1983), Molec. & Cell. Biol., 3:1440-1450.
Fuller et al. in Microbiology 1986, pp. 273-278 (1986).

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