Secretion vector

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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4351723, 435253, 435320, 935 11, 935 14, 935 29, 935 47, 935 48, 935 73, 935 74, 935 75, C12P 2100, C12N 1500, C12N 120, C12N 100

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047693274

ABSTRACT:
A vector including a DNA sequence encoding a secretory signal sequence substantially identical to the secretory signal encoding sequence of the Bacillus licheniformis .alpha.-amylase gene; upstream from the signal-encoding sequence, a promoter sequence and a ribosome binding site sequence, transcription of the signal-encoding sequence being under the control of the promoter sequence; and downstream from the signal-encoding sequence, a site for the insertion into the vector of a heterologous DNA sequence, in reading frame with the signal-encoding sequence.

REFERENCES:
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Inouye, H. et al., J. Bacteriol., vol. 149, No. 2, pp. 434-439, 1982.
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Joyet, P. et al., Fems Microbiology Letters, vol. 21, pp. 353-358, Mar., 1984.
Sibakov et al., Eur. J. Biochem. (1984) 145:567-572.

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