Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi
Patent
1993-09-16
1996-05-28
Vogel, Nancy T.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Fungi
4352523, 43525233, 43525423, 4351723, 435 691, 435 697, 536 234, C12N 119, C12N 121, C12N 1562
Patent
active
055210864
ABSTRACT:
The invention generally features a novel recombinant DNA molecule useful for producing a heterologous protein, e.g., insulin-like growth factor-I, in yeast. The DNA molecule includes the following sequences joined in frame and in the following 5' to 3' progression: a sequence encoding a signal peptide of a yeast secreted protein; a sequence encoding at least 5 amino acids of the N-terminal region of a mature acid phosphatase protein, a sequence encoding a heterologous protein. Preferably the DNA molecule also encodes a spacer element that includes a proteolytic cleavage site.
REFERENCES:
patent: 4546082 (1985-10-01), Kurjan et al.
patent: 4870008 (1989-09-01), Brake
patent: 4963665 (1990-10-01), Rotwein et al.
patent: 5070075 (1991-12-01), Rotwein et al.
Andrews et al., "Sequences beyond the Cleavage Site Influence Signal Peptide Function," The Journal of Biological Chemistry 263:15791-15798, 1988.
Bayne et al., "Expression, purification and characterization of recombinant human insulin-like growth factor I in yeast," Gene 66:234-244, 1988.
Bos et al., "NH.sub.2 -terminal hydrophobic region of influenza virus neuraminidase provides the signal function in translocation," Proc. Natl. Acad. Sci. USA 81:2327-2331, 1984.
Bonatti et al., "Absence of a Cleavable Signal Sequence in Sindbis Virus Glycoprotein PE.sub.2," The Journal of Biological Chemistry 254:12261-12264, 1979.
Chaudhuri et al., "The pro-region of the yeast prepro-.alpha.-factor is essential for membrane-translocation of human insulin-like growth factor 1 in vivo," Eur. J. Biochem. 206:793-800, 1992.
Clare et al., "High-level Expression of Tetanus Toxin Fragment C in Pichia pastoris Strains Containing Multiple Tandem Integrations of the Gene," Bio/Technology 9:455-460, 1991.
Clements et al., "Secretion of human epidermal growth factor from Saccharomyces cerevisiae using synthetic leader sequences," Gene 106:267-272, 1991.
Degryse et al., "Addition of a dipeptide spacer significantly improves secretion of ovine trophoblast interferon in yeast," Gene 118:47-53, 1992.
Kohara et al., "Alteration of N-terminal Residues of Mature Human Lysozyme Affects Its Secretion in Yeast and Translocation into Canine Microsomal Vesicles," The Journal of Biological Chemistry 266:20363-20368, 1991.
Meyack et al., "Two yeast acid phosphatase structural genes . . . in their promoter and coding sequences," The EMBO Journal 6:675-680, 1982.
Palmiter et al., "Ovalbumin: A secreted protein without a transient hydrophobic leader sequence," Proc. Natl. Acad. Sci. USA 75:94-98, 1978.
Sleep et al., "The Secretion of Human Serum Albumin from the Yeast Saccharomyces cerevisiae Using Five Different Leader Sequences," Bio/Technology 8:42-46, 1990.
Strom, et al., "Mapping of Export Signals of Pseudomonas aeruginosa Pilin with Alkaline Phosphatase Fusions," Journal of Bacteriology 169:3181-3188, 1987.
Zsebo et al., "Protein Secretion from Saccharomyces cerevisiae Directed by the Prepro-.alpha.-factor Leader Region," The Journal of Biological Chemistry 261:5858-5865, 1986.
Brierley Russell A.
Howland David S.
Scott Richard W.
Cephalon Inc.
Vogel Nancy T.
LandOfFree
Secretion sequence for the production of a heterologous protein does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Secretion sequence for the production of a heterologous protein , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Secretion sequence for the production of a heterologous protein will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-786030