Secretion plasmid comprising the kilgene

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4351723, 4353201, 4352523, 43525233, 435200, 435209, 530300, 530350, 935 29, 935 41, 935 56, 935 61, 935 73, C12P 2102, C12P 1934, C12N 1500, C12N 121, C07K 300, C07H 1512

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052468399

ABSTRACT:
The present invention provides for a plasmid, pEAP7.DELTA.P, containing a DNA region capable of inducing extracellular secretion of a useful, physiologically active substance in transformed host and a promoter DNA region regulating expression of the first DNA region. The present invention further provides for a microorganism transformed with the plasmid and a process for the production of the substances by culturing the microorganism.

REFERENCES:
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patent: 4948735 (1990-08-01), Luria et al.
Sashihera et al. J. Bacteriol. vol. 158 pp. 5903-506. (1984).
Kobayashi et al. J. Bacteriol. vol. 166, pp. 728-732 (1986).
Kudo et al. J. Bacteriol. vol. 156 pp. 949-951. (1983).
Chan et al. J. Biol. Chem. vol. 260 pp. 8925-8935 (1985).
Sabik et al. J. Bacteriol. vol. 153 pp. 1479-1485 (1983).
Neidhardt, Ed.-in-chief, Escherichia Coli and Salmonello Typhimurium, Cellular and Molecular Biology, vol. 2, pp. 1615-1624 only one re submitted pp. 1615-1619 were considered.

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