Secretion of insulin-like growth factor-I in E. coli

Details Secretion of insulin-like growth factor-I in E. coli Secretion of insulin-like growth factor-I in E. coli

1991-05-23

1996-02-06

Moody, Patricia R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 697, 435 698, 435 712, 935 48, C12N 1570, C12N 1518, C12P 2102

Patent

active

054895170

ABSTRACT:
A method for bacterially producing IGF-I is disclosed in which Gram-negative bacteria are caused to express a gene consisting of a lamb or ompF signal sequence operatively joined to a DNA sequence encoding IGF-I and producing IGF-I which is secreted into the periplasmic space of the bacteria.

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patent: 4738921 (1988-04-01), Belagaje et al.
patent: 5015573 (1991-05-01), Yarranton et al.
Sjostrum et al 1987 (Mar.) EMBO J 6:823-831.
Nilsson et al 1985 Nucleic Acids Research 13:1151-1162.
Nagahari et al 1985 EMBO J. 4:3589-3592.
Hoffman et al 1985 Proc Natl Acad Sci USA 82:5107-5111.
Wong et al 1988 Gene 68:193-203.

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