Secondary metabolite congener distribution modulation

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S118000, C435S252300, C435S252100, C536S023200

Reexamination Certificate

active

10376612

ABSTRACT:
The present invention provides a generalized oxygen-limited cultivation method for myxobacterial strains engineered to heterologously express polyketides synthase (PKS) gene clusters under various oxygen tension conditions, modulating the polyketide congener distribution.

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Pfefferle et al. “Improved secondary metabolite production in the genus Streptosporangium by optimization of fermentation conditions.”J. Biotechnol. (2000) 80: 135-142.
Schneider et al. “Controlled regioselectivity of fatty acid oxidation by whole cells producing cytochrome P450BM-3 monooxygenase under varied dissolved oxygen concentrations.”Biotechnol. Bioengineer. 64: 333-341.

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