Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase
Reexamination Certificate
2008-07-15
2008-07-15
Ramirez, Delia M (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Lyase
C435S320100, C435S252300, C435S325000, C435S069100, C435S410000, C530S350000, C536S023200, C536S023100
Reexamination Certificate
active
10507132
ABSTRACT:
The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion, substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.
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Meinkoth and Wahl, Current Protocols in Molecular Biology, Hybridization Analysis of DNA Blots, pp. 2.10.8-2.10.11, 1993.
Motoyama, T., et al., cDNA cloning, expression, and mutagenesis of scytalone dehydratase needed for pathogenicity of the rice blast fungus, Pyricularia oryzae., Biosci. Biotechnol. Biochem. 1998, vol. 62, No. 3, pp. 564 to 566.
Nakasako, M., et al., Cryogenic X-ray crystal structure analysis for the complex of scytalone dehydratase of a rice blast fungus and its tight-binding inhibitor, carpropamid: the structural basis of tight-binding inhibition., Biochemistry 1998, vol. 37, pp. 9931 to 9939.
Kaku Koichiro
Kawai Kiyoshi
Nagayama Kozo
Shimizu Tsutomu
Watanabe Satoshi
Kumiai Chemical Industry Co. Ltd.
Ramirez Delia M
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