Screening using S. cerevisiae mannosyltransferase encoding genes

Details Screening using S. cerevisiae mannosyltransferase encoding genes Screening using S. cerevisiae mannosyltransferase encoding genes

1999-11-04

2000-11-28

Schwartzman, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 15, 435 29, C12Q 100, C12Q 148, C12Q 102

Patent

active

061533769

ABSTRACT:
The present invention relates to an antifungal in vitro and in vivo screening assays for identifying compounds which inhibit mannosyltransferases involved in protein O- and N-glycosylation. The antifungal screening assay for identifying a compound which inhibit mannosyltransferases involved in protein O- and N-glycosylation, comprises the steps of: a) subjecting proteins to a specific mannosyltransferase protein encoded by a Saccharomyces cerevisiae mannosyltransferase encoding gene, wherein said gene is selected from the group consisting of KRE2/MNT1, YUR1, KTR1, KTR2, KTR3, KTR4, KTR5, KTR6 and KTR7; b) subjecting step a) to a screened compound and determining the absence or presence of protein O- and N-glycosylation, wherein the absence of protein O- and N-glycosylation is indicative of an antifungal compound. There is also disclosed an in vitro method for the diagnosis of diseases caused by fungal infection in a patient.

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