Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1991-10-15
1994-09-20
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 14, 435 723, 435967, 435970, 435975, 436501, 436518, 436 64, 436 94, 436 95, 436813, C12Q 126, C12Q 154, C12Q 100
Patent
active
053488601
ABSTRACT:
An improved screening test method and kit for cancerous and precancerous conditions which is rapid, employs reagents which can be provided in kit form and which does not give false negatives due to sampling error, immobilizes a sample of a proteinaceous body fluid in a membrane filter, tests for marker carbohydrates therein which have vicinal galactose moieties and which are enzymatically oxidized with galactose oxidase to vicinal aldehydic moieties which then visualizes any thus-produced vicinal aldehydic moieties with Schiff's Reagent, then further oxidizes those samples which test negative for the marker carbohydrates with periodic acid, thereby rendering the sample visualizable with Schiff's Reagent if the sample contains glycoprotein, which applies the galactose oxidase directly to the membrane filter, thereby speeding up color developed, and employs Schiff's Reagent which was refrigerated after preparation until its color faded to a straw shade before being treated with activated charcoal, which treatment renders the solution storage-stable for many months and enhances its color development ability.
REFERENCES:
patent: 4857457 (1989-08-01), Shamsuddin et al.
patent: 5162202 (1992-11-01), Shamsuddin
Howard, D. R., et al., "Carcinoma-associated cytostructural antigenic alterations: detection by lectin binding," Cancer, Jun. 15, 1981, 47(12) pp. 2872-2877, Abstract Only.
Kennedy, J. K., in Bioactive Carbohydrates: In Chemistry, Biochemistry and Biology, Ellis Horwood Limited, West Sussex, England, pp. 70-73, 1983.
Fairbanks, G., et al., Biochemistry, vol. 10, No. 13 pp. 2606-2616, 1971.
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