Screening system for identifying compounds that regulate steroid

Details Screening system for identifying compounds that regulate steroid Screening system for identifying compounds that regulate steroid

1995-06-02

1999-08-31

Caputa, Anthony C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 721, 435 71, 435325, 435 78, 4353201, 435 691, 435 694, 435 697, 435975, 536 231, 536 2351, 530350, 530358, C12Q 168, G01N 33566, C12N 1585, C12N 1512

Patent

active

059452790

ABSTRACT:
A tissue culture screening system to monitor a transcriptional response treated by a chemical signal interacting with a plasma membrane receptor is provided. The tissue culture screening system includes a cell line containing a membrane receptor, a target gene and a specific receptor selected from the group consisting of a steroid receptor, a vitamin receptor and an orphan receptor. The specific receptor regulates transcription of the target gene. Any of the target gene membrane receptor or specific receptor can be introduced into the cell by an expression vector. In addition to the screening system there is also provided assays for identifying test compounds and chemical signals that regulate transcription or are potential agonist or antagonist neurotransmitters or which regulate indirectly by a membrane receptor binding or regulate transription in the absence of a steroid, vitamin or orphan ligand. There is further provided kits for the assays.

REFERENCES:
patent: 4981784 (1991-01-01), Evans et al.
Evans Science 240 (1988) 889-895.

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