Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology
Reexamination Certificate
2007-07-31
2007-07-31
Falk, Anne-Marie (Department: 1632)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of regulating cell metabolism or physiology
Reexamination Certificate
active
10157288
ABSTRACT:
This invention provides populations of neural progenitor cells and differentiated neurons, obtained by culturing pluripotent cells in special growth cocktails. The technology can be used to produce progenitors that proliferate through at least ˜40 doublings, while maintaining the ability to differentiate into a variety of different neural phenotypes, including dopaminergic neurons. The neural progenitors and terminally differentiated neurons of this invention can be generated in large quantities for use in drug screening and the treatment of neurological disorders.
REFERENCES:
patent: 5639618 (1997-06-01), Gay
patent: 5672499 (1997-09-01), Anderson et al.
patent: 5766948 (1998-06-01), Gage et al.
patent: 5773255 (1998-06-01), Laurance et al.
patent: 5789246 (1998-08-01), Reid et al.
patent: 5849553 (1998-12-01), Anderson et al.
patent: 5851832 (1998-12-01), Weiss et al.
patent: 5928947 (1999-07-01), Anderson et al.
patent: 5968829 (1999-10-01), Ruddick et al.
patent: 5981165 (1999-11-01), Weiss et al.
patent: 6040180 (2000-03-01), Johe
patent: 6090622 (2000-07-01), Gearhart et al.
patent: 6200806 (2001-03-01), Thomson
patent: 6238922 (2001-05-01), Uchida
patent: 6458589 (2002-10-01), Rambhatla et al.
patent: 6602711 (2003-08-01), Thomson et al.
patent: 6833269 (2004-12-01), Carpenter
patent: 2002/0009743 (2002-01-01), Carpenter
patent: 2002/0012903 (2002-01-01), Goldman et al.
patent: 2002/0151056 (2002-10-01), Sasai et al.
patent: 2004/0023376 (2004-02-01), Thomson et al.
patent: 1022330 (2000-07-01), None
patent: WO 94/03199 (1994-02-01), None
patent: WO 98/50526 (1998-11-01), None
patent: WO 99/01159 (1999-01-01), None
patent: WO 99/04775 (1999-02-01), None
patent: WO 99/20741 (1999-04-01), None
patent: WO 99/28443 (1999-06-01), None
patent: WO 99/43785 (1999-09-01), None
patent: WO 99/53021 (1999-10-01), None
patent: WO 99/53022 (1999-10-01), None
patent: WO 00/17323 (2000-03-01), None
patent: WO 00/47762 (2000-08-01), None
patent: WO 01/51616 (2001-07-01), None
patent: WO 01/68815 (2001-09-01), None
patent: WO 01/83715 (2001-11-01), None
patent: WO 01/98463 (2001-12-01), None
patent: WO 02/081663 (2002-10-01), None
patent: WO 02/086106 (2002-10-01), None
patent: WO 2004/007696 (2004-01-01), None
Lodish, ed., et al. (2000) Molecular Cell Biology, 4thEdition. (W.H. Freeman, New York), p. 968.
Schuldiner et al. (2000) Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. USA 97(21): 11307-11312.
Verfaillie et al. (2002) Stem cells: Hype and reality. 2002: 369-391.
Andrews, et al., Retinoic Acid Induces Neuronal Differentiation of a Cloned Haman Embryonal Carcinoma Cell Line in Vitro, Dev. Biol. 103:285 (1984).
Bain, et al., Embryonic Stem Cells Express Neuronal Properties in Vitro, Dev. Biol. 168:342 (1995).
Bain, et al., Retinoic Acid Promotes Neural and Represses Mesodermal Gene Expression in Mouse Embryonic Stem Cells in Culture, Chem. and Biophys. Res. Comm. 223:691 (1996).
Bain, et al., Neural Cells Derived by In Vitro Differentiation of P19 and Embryonic Stem Cells, Perspectives Dev. Neurobio. 5:175 (1998).
Bodnar, et al., Extension of Life-span by Introduction of Telomerase into Normal Human Cells, Science 279:349 (1998).
Brustle, et al., In Vitro-Generated Neural Precursors Participate in Mammalian Brain Development, Proc. Natl. Acad. Sci. USA 94:14809 (1997).
Brustle, et al., Embryonic Stem Cell-Derived Glial Precursors: A Source of Myelinating Transplants, Science 285:754 (1999).
Clarke, et al., Generalized Potential of Adult Neural Stem Cells, Science 288:1660 (2000).
Deacon, et al., Blastula-Stage Stem Cells Can Differentiate into Dopaminergic and Serotonergic Neurons after Transplantation, Exp. Neurol. 149:28 (1998).
Fraichard, et al., In Vitro Differentiationof Embryonic Stem Cells into Glial Cells and Functional Neurons, J. Cell Science 108:3181 (1995).
Kalyani, et al., Cell Lineage in the Developing Neural Tube, Biochem. Cell Biol. 76:1051 (1998).
Lee, et al., Efficient Generation of Midbrain and Hindbrain Neurons from Mouse Embryonic Stem Cells, Nat. Biotechnol. 18:675 (2000).
Li, et al., Generation of Purified Neural Precursors from Embryonic Stem Cells by Lineage Selection, Curent Biology 8:971.
Ling, et al., Differentiation of Mesencephalic Progenitor Cells into Dopaminergic Neurons by Cytokines, Exp. Neurol. 149:411 (1998).
Liu, et al., Embryonic Stem Cells Differentiate into Oligodendrocytes and Myelinate in Culture and After Spinal Cord Transplantation, PNAS 97:6126 (2000).
Mayer-Prosche, et al., Isolation of Lineage-Restricted Neuronal Precursors from Mltipotent Neuroepithelial Stem Cells, Neuron 19:773 (1997).
McDonald, et al., Transplanted Embryonic Stem Cells Survive, Differentiate and Promote Recovery in Injured Rat Spinal Cord, Nat. Med. 5:1410 (1999).
Mujtaba, et al., Lineage-Restricted Neural Precursors Can Be Isolated from Both the Mouse Neural Tube and Cultured ES Cells, Dev. Biol. 214:113 (1999).
Okabe, et al., Development of Neuronal Precursor Cells and Funcitonal Postmitotic Neurons from Embryonic Stem Cells in Vitro, Mechanisms of Dev. 59:89 (1996).
Reubinoff, et al., Embryonic Stem Cell Lines From Human BlastoCysts: Somatic Differentiation In Vitro, Nature Biotechnol. 18:399 (2000).
Shamblott, et al., Derivation of Pluripotent Stem Cells From Cultured Human Primordial Germ Cells, Proc. Natl. Acad. Sci. USA 95:13726 (1998).
Strubing, et al., Differentiationof Pluripotent Embryonic Stem Cells into the Neuronal Lineage in Vitro Gives Rise to Mature Inhibitory and Excitatory Neurons, Mechanisms of Dev. 53″275 (1995).
Thomson, et al., Neural Differentiation of Rhesus Embryonic Stem Cells, APMIS 106:149 (1998).
Thomson, et al., Embryonic Stem Cell Lines Derived from Human Blastocysts, Science 282:1145 (1998).
Tropepe, et al., Autonomous Neural Cell Fate Specification in Mouse Embryonic Stem Cells—Abstract, Society for Neuroscience 25:527 (1999).
van Inzen, et al., Meuronal Differentiation of Embryonic Stem Cells, Biochimica et Biophsica Acta 1312:21 (1996).
Wagner, et al., Induction of a Midbrain Dopaminergic Phenotype in Nurr1-overexpressing Neural Stem Cells by Type 1 Astrocytes, Nature Biotechnol. 17:653 (1999).
Yao, et al., Neuronal Differentiation of P19 Embryonal Carcinoma cells in Defined Media, J. Neuroscience Res. 41:792 (1995).
Neural Implant Technologies, Neurolnvestment (Dec. 1999).
Lamb, T.M., et al., Neural Induction by the Secreted Polypeptide Noggin, Science 262:713 (1993).
Lim, D.A., et al., Noggin Antagonizes BMP Signaling to Create a Niche for Adult Neurogenesis, Neuron 27:713 (2000).
Sasal, Y., et al., Regulation of Neural Induction by the Chd and Bmp-4 Antagonistic Patturning Signals in Zenopus Nature 376:333 (1995).
Kalyani, A., et al., Cell Lineage in the Developing Neural Tube, Biochem. Cell Biol. 76:1051 (1998).
Li, M., et al., Generation of Purified Neural precursors from Embryonic Stem Cells by Lineage Selection, Current Biol., Current Science 8:971 (1998).
Mujtaba, T., et al., Lineage-Restricted Neural Precursors Can Be Isolated from Both the Mouse Neural Tube and Cultured ES Cells, Dev. Biol. 214:113 (1999).
Juul, et al., Erythropoietin and Erythropoietin Receptor in the Developing Human Central Nervous System, Pediatric Research 43:40 (1998).
Erythropoietin Regulates the In Vitro and In Vivo Production of Neuronal Progenitors by Mammalian Forebrain Neural Stem Cells, J. Neuroscience 21:9733 (2001).
Studer, L., et al., Enhanced Proliferation, Survival, and Dopaminergic Differentiation of CNS Precursors in Lowered Oxygen, J. Neuroscience 20:7377 (2000).
Kawasaki, et al., Generation of dopaminergic neurons and pigmented epithelia from primate IS cells by stromal cell-derived inducing activity, PNAS 99:1580 (2002).
Bain G et al, Expression of Retinoid X Receptors in P19 Embryonal Carcinoma Cells and Embryonic Stem Cells, Biochem Biophys Res Comm 200(3):1252 (1994).
Biesecker LG et al, Interleukin-6 is a Component of Human Umbilical Cord Serum and Stimulates Hematopoiesis in Embryonic Stem Cells in Vitro, Exp Hematol 21:774 (
Carpenter Melissa K.
Denham Jerrod J.
Inokuma Margaret S.
Thies R. Scott
Falk Anne-Marie
Geron Corporation
Wise Bart W.
LandOfFree
Screening small molecule drugs using neural cells... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Screening small molecule drugs using neural cells..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Screening small molecule drugs using neural cells... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3750260