Screening procedure for inhibitors of HIV Rev function

Details Screening procedure for inhibitors of HIV Rev function Screening procedure for inhibitors of HIV Rev function

1997-07-11

2000-05-02

Budens, Robert D.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 436501, 536 234, 536 2372, C12Q 170, C12Q 168

Patent

active

060570954

ABSTRACT:
This invention provides a method of screening for inhibitors of HIV Rev function, comprising introducing into a cell a nucleic acid construct comprising a reporter gene positioned in the construct such that expression of the reporter gene increases when Rev function decreases and wherein the cell contains Rev, administering to the cell a potential inhibitor of Rev function, monitoring the expression of the reporter gene, and correlating the expression of the reporter gene to an inhibition of Rev function. Also provided is a method of screening for inhibitors of HIV Rev function, further comprising introducing into a second cell a second nucleic acid construct comprising a reporter gene wherein Rev does not affect the expression of the reporter gene of the second nucleic acid construct, administering to the cells a potential inhibitor of Rev function, monitoring the expression of the reporter genes, correcting the expression of the reporter gene in the first nucleic acid construct by the expression of the reporter gene in the second nucleic acid construct, and correlating the corrected expression of the reporter gene in the first nucleic acid construct to an inhibition of Rev function. The invention also provides a method for monitoring expression of regulatory genes of HIV relative to structural genes, a method of screening for inhibition of the function of a Rev-responsive element (RRE), and isolated nucleic acid constructs comprising an HIV RRE and a reporter gene positioned such that expression of the reporter gene decreases when Rev protein interacts with the RRE.

REFERENCES:
patent: 5534408 (1996-07-01), Green et al.
Ciccarelli etal., Antiviral Chem. Chemother. 5(3): 169-175, 1994.
Arrigo et al. "Analysis of rev gene function on human immunodeficiency virus type 1 replication in lymphoid cells by using a quantitative polymerase chain reaction method" J. Virol. 63:4875-4881, 1989.
Arrigo et al. "Rev is necessary for translation but not cytoplasmic accumulation of HIV-1 vif, vpr, and env/vpu 2 RNAs." Genes Dev. 5:808-819, 1991.
Raghavendar et al. "Identification and mapping of inhibitory sequences in the human immunodeficiency virus type 2 vif gene" J. Virol. 69:5167-5170, 1995.
Cochrane et al. "Identification and characterization of intragenic sequences which repress human immunodeficiency virus structural gene expression" J. Virol. 65:5305-5313, 1991.
Burki, K. "Genetically engineered cells and animals: new tools in pharmacology" Int. Pharm. J. 9:Suppl.1,4.
Shukla et al. "Human chromosome 6- and 11-encoded factors support human immunodeficiency virus type 1 function in A9 cells" J. Virol. 70:9064-9068, 1996.
Schiller et al. "Rapid complementation assay for anti-HIV-1 drug screening and analysis of envelope protein function" Aids Res. Hum. Retro. 8:1723-1731, 1992.
Helseth et al. "Rapid complementation assays measuring replicative potential of human immunodeficiency virus type 1 envelope glycoprotein mutants" J. Virol. 64:2416-2420, 1990.
Bahner et al. "Comparison of trans-dominant inhibitory mutant human immunodeficiency virus type 1 genes expressed by retroviral vectors in human T lymphocytes" J. Virol. 67:3199-3207, 1993.
Xiaobin et al. "Assay systems for HIV Rev function using chimeric gene constructions" V Int. Conf. on AIDS, Jun. 4-9 (1262p) 1989.

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