Chemistry: analytical and immunological testing – Measurement of electrical or magnetic property or thermal... – Of a liquid
Reexamination Certificate
2006-02-07
2006-02-07
Wang, Andrew (Department: 1639)
Chemistry: analytical and immunological testing
Measurement of electrical or magnetic property or thermal...
Of a liquid
C436S149000, C435S004000, C435S007100, C435S007200
Reexamination Certificate
active
06995022
ABSTRACT:
A high throughput, on-line, pulsed ultrafiltration-mass spectrometric method has been developed to determine whether a compound has predetermined characteristics that would make it suitable for a specific purpose, e.g. drug development. The method is useful to generate, identify, and quantify metabolites of compounds formed by drug metabolizing enzymes such as cytochrome P450, UDP-glucuronyltransferases, and glutathione transferases. The method is useful for rapid screening of drugs or other compounds to determine the extent of their metabolism and to characterize their primary metabolites. If reactive and potentially toxic metabolites are formed during, e.g. cytochrome P450 oxidation, the metabolites can be reacted with glutathione and then detected on-line using mass spectrometry in a rapid assay to assess the potential for toxicity. In addition, the method is useful for the determination of bioavailability, absorption and cell permeability of compounds.
REFERENCES:
patent: 5366862 (1994-11-01), Venton et al.
patent: 5478723 (1995-12-01), Parkinson et al.
patent: 5567592 (1996-10-01), Benet et al.
patent: 5872015 (1999-02-01), Venton et al.
van Breemen et al., “Metabolic Screening Using On-Line Ultrafiltration Mass Spectrometry”, Feb. 1998, Drug Metabolism and Disposition, 26(2):85-90.
van Breemen et al., Pulsed Ultrafiltration Mass Spectrometry: A New Method for Screening Combinatorial Libraries, Anal. Chem., Jun. 1, 1997, 69(11): 2159-2164.
Zhao et al., Screening Solution-Phase Combinatorial Libraries Using Pulsed Ultrafiltration/Electrospray Mass Spectrometry, J. Med. Chem., Dec. 5, 1997, 40(25):4006-4012.
Wieboldt et al. 1997. Anal Chem 69:1683-1691.
Stevanovic et al. 1993. Bioorganic & Medicinal Chemistry Letters. 3:431-436.
Metzger et al. Angew Chem Int Ed Engl. 32:985-896.
Hidalgo, I.J., et al. (1989) “Characterization of the Human Colon Carcinoma Cell Line (Caco-20) as a Model System For Intestinal Epithelial Permeability,”Gastroenterology96: 736-749.
Parkinson, A., et al. (1996) “An Overview of Current Cytochrome P450 Technology for Assessing the Safety and Efficacy of New Materials,”Toxicol Pathol24: 45-57.
Bolton Judy L.
van Breemen Richard B.
Barnes & Thornburg LLP
Board of Trustees of the University of Illinois
Martin Alice O.
Tran My-Chau T
Wang Andrew
LandOfFree
Screening of xenobiotics and endogenous compounds for... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Screening of xenobiotics and endogenous compounds for..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Screening of xenobiotics and endogenous compounds for... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3663930