Screening of candidates for biological hair care activity

Drug – bio-affecting and body treating compositions – Live hair or scalp treating compositions – Polymer containing

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424 941, 424 9467, 424543, 514880, 435 1, 435 701, 4352402, 43524021, 43524022, A61K 706

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active

058914269

ABSTRACT:
The invention concerns a procedure for testing a potentially-active substance used in the hair-care field, this procedure being characterized by the fact that a fragment of at least one hair follicle without dermal papilla is isolated, this fragment being taken from beneath the point of attachment of the sebaceous gland and excluding the portion of the hair shaft located above the point of attachment of the sebaceous gland; that this fragment is incubated in a suitable culture medium for a sufficient period; that this fragment is placed in contact with a potentially-active hair-care substance; that a marker of the activity of said tested substance is quantitatively determined; and that the results of this determination are assessed as they compare to a control.

REFERENCES:
patent: 5667961 (1997-09-01), Bernard et al.
Journal of Ivestigative Dermatology, vol. 105, No. 2, Aug. 1995, New York, N.Y., pp. 177-183, XP000196475 Scandurro et al: "Immortalized rat whisker dermal papilla cells cooperate with mouse immature hair follicle buds to activate type IV procollagenases in collagen matrix coculture: Correlation with ability to promote hair follicle development in nude mouse grafts."
Journal of Investigative Dermatology, vol. 101, No. 1 (Supplement), Jul. 1993, New York, N.Y., pp. 27S-32S, XP000196474 Yuspa et al: "Regulation of hair follicle development: an in vitro model for hair follicle invasion of dermis and associated connective tissue remodeling."
SIGMA chemical company catalog, p. 275 (showing different types of collagenases), 1992.

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