Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-06-12
1998-07-21
Feisee, Lila
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 4, 435 5, 435 6, 435 78, 435 79, 435 72, 204299, 2041801, 204407, 356344, 436 6, G01N 3353, G01N 3300, G01N 2100, C25D 1300
Patent
active
057833974
ABSTRACT:
A method in which natural sample components are simultaneously fractionated and screened for compounds that bind tightly to specific molecules of interest is disclosed. Such newly isolated ligands are good candidates for potential therapeutic or diagnostic compounds. The natural sample is first combined with a potential target molecule and then subjected to capillary electrophoresis (CE). Charged (or even neutral) compounds present in the natural sample that bind to the added target molecule can alter its normal migration time upon CE, by changing its charge-to-mass ratio, or will cause a variation in peak shape or area. Complex formation can be detected by simply monitoring the migration of the target molecule during electrophoresis. Any new ligands that bind to the target molecule will be good candidates for therapeutic or diagnostic compounds. Interfering, weak-binding ligands commonly present in crude extracts are not detected. Small, neutral ligands, as well as charged ligands, can be identified in competitive binding experiments with known, charged competitor molecules.
REFERENCES:
patent: 5234586 (1993-08-01), Afeyan et al.
patent: 5324401 (1994-06-01), Yeung et al.
patent: 5431793 (1995-07-01), Wang et al.
patent: 5532124 (1996-07-01), Block et al.
patent: 5534410 (1996-07-01), Tjian et al.
patent: 5536382 (1996-07-01), Sunzeri
patent: 5567282 (1996-10-01), Wang et al.
Yen-Ho Chu et al., "Affinity Capillary Electrophoresis-Mass Spectrometry for Screening Combinatorial Libraries", J. Am. Chem. Soc. vol. 118:7827-7835 (1996).
Chu et al., "Using Affinity Capillary Electrophoresis To Identify the Peptide in a Peptide Library that Binds Most Tightly to Vancomycin," J. Org. Chem. 58:648-652 (1993).
Chu et al., "Using Affinity Capillary Electrophoresis To Determine Binding Stoichiometries of Protein-Ligand Interactions," Biochemistry 33:10616-10621 (1994).
Chu et al., "Affinity Capillary Electrophoresis," Acc. Chem. Res. 28:461-468 (1995).
Crothers, "Gel electrophoresis of protein-DNA complexes," Nature 325:464-465 (1987).
Ceglarek et al., "Studies of DNA-protein interactions by gel electrophoresis," Electrophoresis 10:360-365 (1989).
Fried et al., "Measurement of protein-DNA interaction parameters by electrophoresis mobility shift assay," Electrophoresis 10:366-376 (1989).
Xian et al., "DNA-protein binding assays from a single sea urchin egg: A high-sensitivity capillary electrophoresis method," Proc. Natl. Acad. Sci. USA 93:86-90 (1996).
Heegaard et al., "Use of Capillary Zone Electrophoresis To Evaluate the Binding of Anionic Carbohydrates to Synthetic Peptides Derived from Human Serum Amyloid P Component," Anal. Chem. 64:2479-2482 (1992).
Wagner et al., "Use of Affinity Capillary Electrophoresis To Measure Binding Constants of Ligands to Proteins," J. Med. Chem. 35:2915-2917 (1992).
Shimura et al., "Affinity Probe Capillary Electrophoresis: Analysis of Recombinant Human Growth Hormone with a Fluorescent Labeled Antibody Fragment," Anal. Chem. 66:9-15 (1994).
Sun et al., "Enhanced albumin protein separations and protein-drug binding constant measurements using anti-inflammatory drugs as run buffer additives in affinity capillary electrophoresis," Journal of Chromatography 661:335-340 (1994).
Heegard, "Determination of antigen-antibody affinity by immuno-capillary electrophoresis," Journal of Chromatography 680:405-412 (1994).
Liu et al., "Affinity capillary electrophoresis applied to the studies of interactions of a member of heat shock protein family with an immunosuppressant," Journal of Chromatography 680:395-403 (1994).
Pritchett et al., "Capillary Electrophoresis-Based Immunoassays," Bio/Technology 13:1449-1450 (1995).
Koutny et al., "Microchip Electrophoretic Immunoassay for Serum Cortisol," Anal. Chem. 68:18-22 (1996).
Chu et al., "Free Solution Identification of Candidate Peptides from Combinatorial Libraries by Affinity Capillary Electrophoresis/Mass Spectrometry," J. Am. Chem. Soc. 117:5419-5420 (1995).
Z. Deyl et al, J. Chromatogr (1994) 656(1) 3-27.
Chu et al., J. Med. Chem, (1992) 35:2915-2917.
Dent & Latchman, DNA mobility Shift Assay in Transcription Factors, Oxford Univ Press, Oxford pp. 1-26.
Ueno & Yeung, Analytical Chem, (1994) 66: 1424-1431.
Perkins & Tomer, Analytical Chemistry, 1994, 66: 2835-2840.
Avila et al, J. Med. Chem, 1993, 36: 126-133.
Lakin, Transcription Factors, Oxford Univ. Press, Oxford, pp. 27-29.
Gordon et al, Analytical Chem., 1994 66: 2835-2840.
Mayer et al, Anal Chem, 1994, 66:1777-1780.
Schmalzing, Anal Chem, 67: 606-612, 1995.
Hughes Dallas E.
Karger Barry L.
Feisee Lila
Northeastern University
Ungar Susan
LandOfFree
Screening natural samples for new therapeutic compounds using ca does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Screening natural samples for new therapeutic compounds using ca, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Screening natural samples for new therapeutic compounds using ca will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1645909