Screening methods in eucaryotic cells

Details Screening methods in eucaryotic cells Screening methods in eucaryotic cells

1997-04-01

1999-11-23

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 4351722, 4351723, 435240, 435 731, 435 70, 43525421, 536 231, 536 241, 536 234, 536 243, C12Q 168, C12N 119, C12N 1563, C07H 2102

Patent

active

059898143

ABSTRACT:
The invention provides new methods for screening libraries of peptides and other compounds for a desired property in eucaryotic cells. The methods are premised, in part, on the unexpected observation that the contents of procaryotic or lower eucaryotic cells, such as yeast, can be transferred to recipient eucaryotic cells in an essentially clonal manner by protoplast fusion of the respective cells. Applications of the methods include screening peptides in eucaryotic cells substantially incapable of episomal replication of transferred nucleic acid fragments; screening in eucaryotic cells peptides or secondary metabolites produced in procaryotic cells; and screening a library of peptides for capacity to bind a selected RNA in eucaryotic cells.

REFERENCES:
patent: 4608339 (1986-08-01), Yoakum et al.
patent: 5610015 (1997-03-01), Wickens et al.
patent: 5637463 (1997-06-01), Dalton et al.
patent: 5683899 (1997-11-01), Stuart
patent: 5695941 (1997-12-01), Brent et al.
Lufkin et al, "Identification by cell fusion of gene sequences that interact with positive trans-acting factors", Science 237:283-286, Jul. 1987.
Nonet et al, "Introduction of YACs containing a putative mammalian replication origin into mammalian cells can generate structures that replicate autonomously", Somatic Cell and Molecular Genetics 19(2):171-192, 1993.
Allen James et al., "Finding Prospective Partners in the Library: the Two-Hybrid System and Phage Display Find a Match," in Trends Biochem. Sci., vol. 20, pp. 511-516, (Dec. 1995).
Felgner Philip L. et al., "Lipofection: A Highly Efficient, Lipid-Mediated DNA-Transfection Procedure," in Proc. Natl. Acad. Sci., USA, Biochemisty part, vol. 84, pp. 7413-7417, (Nov. 1987).
Fouts Derrick E. et al., "Improved Method for Selecting RNA-Binding Activities in Vivo," in Nucleic Acids Res, vol. 24, No. 8, pp. 1582-1584, 1996.
Harada Kazuo et al., "Selection of RNA-Binding Peptides in Vivo," in Nature, vol. 380, pp. 175-179, (Mar. 14, 1996).
Jain CHaitanya et al., "A Structural MO&1 for the HIV-1 Rev-RRE Complex Deduced from Altered Specificity Rev Variants Isolated by a Rapid Genetic Strategy," in Cell, vol. 87, pp. 115-125, (Oct. 4, 1996).
Kinsella Todd M. et al., "Episomal Vectors Rapidly and Stably Produce High-Titer Recombinant Retrovirus," in Human Gene Therapy, vol. 7, pp. 1405-1413, (Aug. 1, 1996).
Kitamura Toshio et al., "Efficient Screening of Retroviral cDNA Expression Libraries," Proc. Natl. Acad. Sci., USA, Biochemistry part, vol. 92, pp. 9146-9150, (Sep. 1995).
Rassoulzadegan Minoo et al., "High Frequency of Gene Transfer After Fusion Between Bacteria and Eukaryotic Cells," in Nature, vol. 295, pp. 257-259, (Jan. 21, 1982).
Sandri-Goldin Rozanne et al., "High-Frequency Transfer of Cloned Herpes Simplex Virus Type 1 Sequences to Mammalian Cells by Protoplast Fusion," in Molecular and Cellular Biology, vol. 1, No. 8, pp. 743-752, (Aug. 1981).
Schaffner Walter, "Direct Transfer of Cloned Genes From Bacteria to Mammalian Cells," in Proc. Natl. Acad. Sci., USA, vol. 77, No. 4, Genetics part, pp. 2163-2167, (Apr. 1980).
Seed Brian et al., "Molecular Cloning of the CD2 Antigen, the T-Cell Erythrocyte Receptor, by a Rapid Immunoselection Procedure," in Proc. Natl. Acad. Sci., USA, vol. 84, Immunology part, pp. 3365-3369, (May 1987).
Seed Brian, "Developments in Expression Cloning," in Curr. Op. Biotechnol., vol. 6, Current Opinion in Biology, pp. 567-573, 1995.
Wilhelm et al., "A One-Hybrid System for Detecting RNA-Protein Interactions," in Genes to Cells, vol. 1, pp. 317-323, 1996.
Yates John L. et al., "Stable Replication of Plasmids Derived from Epstein-Barr Virus in Various Mammalian Cells," in Nature, vol. 313, pp. 812-815, (Feb. 28, 1985).
Li et al, "Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system", Science 262:1870-1874, Dec. 1993.
Wilhelm et al, "A one-hybrid system for detecting RNA-protein interactions", Genes to Cells, 1(3):317-23 Abstract only, Mar. 1996.

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