Screening methods for the identification of novel antibiotics

Details Screening methods for the identification of novel antibiotics Screening methods for the identification of novel antibiotics

1995-09-15

1998-05-05

Leary, Louise

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 17, 435 23, 435 35, 435 31, 435 4, 435 72, 435 771, 435 24, 435 692, 435176, 435177, 435968, 530300, C12Q 118, C12Q 150, C12Q 116, G01N 3353

Patent

active

057472769

ABSTRACT:
The present invention relates to screening methods for the identification of compounds and compositions useful as novel antibiotics and antibacterial agents. In particular, the present invention relates to methods utilizing two-component regulatory switches, for example, those comprising a prokaryotic enzyme such as histidine protein kinase that is activated to autophosphorylate by a signal transduction mechanism. The invention also relates to methods of identifying inhibitors of enzyme activity, particularly in bacterial cells. In particular, a high-throughput assay system useful in the large-scale screening of protein kinase inhibitors is disclosed.

REFERENCES:
Burbulys, et al., Cell 64: 545-552 (1991) Month Not Available Please Print.
Perego, et al., J. Bacteriol. 171: 6187-6196 (1989) Month Not Available.
Satola, et al., Proc. Natl. Acad. Sci., USA 88: 4533-4537 (1991) Month Not Available.
Strauch, et al., Embo J. 8: 1615-1621 (1989) Month Not Available.
Strauch, et al., Proc. Natl. Acad. Sci., USA 87: 1801-1805 (1990) Month Not Available.
Trach, et al., Res. Microbiol. 142: 815-823 (1991) Month Not Available.
Trach, et al., Molec. Microbiol. 8: 69-79 (1993) Month Not Available.

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