Screening methods for identifying viral proteins with...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

Reexamination Certificate

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C435S029000, C435S032000, C435S235100, C435S006120

Reexamination Certificate

active

07060430

ABSTRACT:
The present invention relates, in general, to a screening method for identifying novel viral proteins with interferon antagonizing function using a transfection-based assay, and the use of such proteins in isolating various types of attenuated viruses for the development of vaccine and pharmaceutical formulations. The invention also relates to the use of viral interferon antagonists in screening assays to identify potential anti-viral agents. The invention further relates to protocols utilizing interferon antagonists, e.g., NS1, to enhance gene therapy or DNA vaccination based on their ability to increase gene expression.

REFERENCES:
patent: 6326151 (2001-12-01), Katze et al.
patent: 100 20 505 (2001-10-01), None
patent: WO 97/08292 (1997-03-01), None
patent: WO 99/64068 (1999-12-01), None
patent: WO 99/64570 (1999-12-01), None
patent: WO 99/64571 (1999-12-01), None
Beattie et al., 1995, “Reversal of the Interferon-Sensitive Phenotype of a Vaccinia Virus Lacking E3L by Expression of the Reovirus S4 Gene,” J. Virology 69(1):499-505.
Bossert et al., 2002, “Respiratory Syncytial Virus (RSV) Nonstructural (NS) Proteins as Host Range Determinants: a Chimeric Bovine RSV with NS Genes from Human RSV Is Attenuated in Interferon-Competent Bovine Cells”, J. of Virology 76:4287-93.
Butterfield and Campbell, 1978, “Vaccination for Fowl Plague”, Selected Reports and Notes, 671-674.
Clemens and Elia, 1997, “The Double Stranded RNA-Dependent Protein Kinase PKR: Structure and Function”, Journal of Interferon and Cytokine Research, 17:503-524.
Didcock et al., 1999, “The V Protein of Simian Virus 5 Inhibits Interferon Signalling by Targeting STAT1 for Proteasome-Mediated Degradation”, J. of Virology, 73(12): 9928-9933.
Floyd-Smith et al., 1981, “Interferon Action: RNA Cleavage Pattern of a (2′-5′) Oligoadenylate-Dependent Endonuclease”, Science 212: 1030-1032.
Garcia-Sastre et al., 1998, “The Role of Interferon in Influenza Virus Tissue Tropism”, J. of Virology, 72(11): 8550-8558.
Garcia-Sastre et al., 1998, “Infuenza A Virus Lacking the NS1 Gene Replicates in Interferon-Deficient System”, Virology 252:324-330.
Garcin et al., 1999, “Sendai Virus C Proteins Counteract the Interferon-Mediated Induction of an Antiviral State”, J. Virology 73(8): 6559-6565.
Gotoh et al., 1999, “Knockout of the Sendai Virus C Gene Eliminates the Viral Abiliity to Prevent the Interferon-α/β-Mediated Responses”, FEBS Letters 459:205-210.
Haller et al., 1998, “Mx Proteins: Mediators of Innate Resistance to RNA Viruses”, Rev. Sci. Tech. Off. Int. Epiz., 17(1):220-230.
Hatada and Fukuda, 1992, “Binding of influenza A virus NS1 protein to dsRNA in vitro”, J. of General Virology, 73: 3325-3329.
He et al., 1997, “The 34.5 Protein Of Herpes Simplex Virus 1Complexes With Protein Phosphatase 1 α to Dephosphorylate the α subunit of the eukaryotic translation initiation factor 2 and preclude the shutoff of protein synthesis by double stranded RNA-activated protein kinase”, Proc. Natl. Acad. Sci. USA, 94:843-848.
Komatsu et al., 2000, “Sendai Virus Blocks Alpha Interferon Signaling To Signal Transducers and Activators of Transcription”, J. Virology, 74(5): 2477-2480.
Lu et al., 1995, “Binding of the Influenza Virus NS1 Protein to Double Stranded RNA Inhibits the Activation of the Protein Kinase that Phosyphorylates the elF-2 Translational Factor”, Virology 214:22-228.
Naniche et al., 2000, “Evasion of Host Defenses by Measles Virus: Wild Type Measles Virus Infection Interferes with Induction of Alpha/Beta Interferon Production”, J. Virology, 74(16):7478-7484.
Norton et al., 1987, “Infectious Influenza A and B Virus Variants with Long Carboxyl Terminal Deletions in the NS1 Polypeptides”, Virology 156:204-213.
Schlender et al., 2000, “Bovine Respiratory Syncytial Virus Nonstructural Proteins NS1 and NS2 Cooperatively Antagonize Alpha/Beta Interferon-Induced Antiviral Response”, J. of Virology 74(18):8234-8242.
Stark et al., 1998, “How Cells Respond to Interferons”, Annu.Rev.Biochem. 67:227-264.
Talon et al., 2000, “Activation of Interferon Regulatory Factor 3 Is Inhibited by the Influenza A Virus NS1 Protein”, J. Of Virology, 74(17): 7989-7996.
Talon et al., 2000, “Influenza A and B Viruses Expressing Altered NS1 Proteins: A vaccine Approach”, Proc. Natl. Acad. Sci USA 97(8):4309-4314.
Wang et al., 2000, “Influenza A Virus NS1 Protein Prevents Activation of NF-KB and Induction of Alpha/Beta Interferon”, J. of Virology 74(24): 11566-11573.
Yoshida et al., 1981, “Characterization of the RNA Associated with Influenza A Cytoplasmic Inclusions and the Interaction of NS1Protein with RNA”, Virology 110: 87-97.
Young et al., 2000, “Paramyxoviridae Use Distinct Virus Specific Mechanisms to Circumvent the Interferon Response”, Virology, 269:383-390.

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