Screening methods for identifying ligands

Data processing: measuring – calibrating – or testing – Measurement system in a specific environment – Chemical analysis

Reexamination Certificate

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C435S006120, C435S007100, C117S011000

Reexamination Certificate

active

06950757

ABSTRACT:
This invention relates to crystallization based assays for identifying ligands that bind to a macromolecule.

REFERENCES:
patent: 5130105 (1992-07-01), Carter et al.
patent: 6039804 (2000-03-01), Kim et al.
patent: 6130227 (2000-10-01), Merlini et al.
patent: 6267935 (2001-07-01), Hol et al.
patent: 6297021 (2001-10-01), Nienaber et al.
patent: WO 99/45379 (1999-09-01), None
patent: WO 01/88113 (2001-11-01), None
Bernstein B., et al., “A bisubstrate analog induces unexpected conformational changes in phosphoglycerate kinase fromTrypanosoma brucei,” Journal of Molecular Biology, vol. 279, p. 1137-1148 (1998).
Bernstein B., et al., “Synergistic effects of substrate-induced conformational changes in phosphoglycerate kinase activation,”Nature, vol. 385 p. 275-278 (1997).
Brünger A, et al., “Crystallography and NMR systems (CNS) A new software system for macromolecular structure determination,”Acta Crystallographica, Section D, vol. D54, p. 905-921 (1998).
Burgin A., et al., “A novel suicide substrate for DNA topoisomerases and site-specific recombinases,”Nucleic Acids Research, vol. 23, p. 2973-2979 (1995).
Champoux J., “DNA Topoisomerases: structure, function, and mechanism,”Annual Reviews Biochem, vol. 70 p. 369-413, (2001).
Chen A., et al., “DNA topoisomerases: Essential enzymes and lethal targets,”Annu. Rev. Pharmacol. Toxicol, vol. 34, p. 191-218 (1994).
Hampton Research Solutions for Crystal Growth, Crystal Screen Reagent Formulations, listing PDF, 3pp, Downloaded from Hampton Research Website http://www.hamptonresearch.com/hrproducts/2110.html on World Wide Web URL: http://www.hamptonresearch.com/hrproducts/2110.html.
Hertzberg R., et al., “On the mechanism of topisomerase I inhibition by camptothecin: evidence for binding to an enzyme-DNA complex,”Biochemistry, vol. 28 p. 4629-4638 (1989).
Hsiang Y., et al., “Camptothecin induces protein-linked DNA breaks via mammalian DNA Topoisomerase I.”The Journal of Biological Chemistry, vol. 260, p. 14873-14878, (1985).
Huang H., et al., “Structure of a covalently trapped catalytic complex of HIV-1 reverse transcriptase: implications for drug resistance,”Science(1998), 282, p. 1669-1675.
Jia Z., et al., “Structure of protein tyrosine phosphatase 1B in complex with inhibitors bearing two phosphotyrosine mimetics,”Journal of Medicinal Chemistry, vol. 44, p. 4584-4594 (2001).
McRee D., “XtalView/Xfit—A Versatile Program for Manipulating Atomic Coordinates and Electron Density,”Journal of Structural Biology, vol. 125, p. 156-165 (1999).
Navaza J., “AMORE: a automated package for molecular replacement,”Acta. Crystallogrphica, vol. A50, p. 157-163 (1994).
Nitiss J., et al., “DNA topoisomerase-targeting antitumor drugs can be studied in yeast,”Proc. Natl. Acad. Sci. U.S.A., vol. 85, p. 7501-7505 (1988).
Redinbo M., et al., “Crystal structures of human topoisomerase l in covalent and noncovalent complexes with DNA,” Science, vol. 279, p. 1504-1513 (1998).
Scapin G., et al., “The structure of apo protein-tyrosine phosphatase 1B C215S mutant: more than just an S→ O change,”Protein Science, vol. 10, p. 1596-1605 (2001).
Stewart L., et al., “Reconstitution of human topoisomerase l by fragment complementation.”J Mol Biol, vol. 269, p. 355-372 (1997).
Stewart L., et al., “High-throughput crystallization and structure determination in drug discovery,”Drug Discovery Today, vol. 7, 187-196 (2002).
Stewart L., et al., “Biochemical and biophysical analyses of recombinant forms of human topoisomerase l,”The Journal of Biological Chemistry, vol. 271, p. 7593-7601 (1996).
Stewart L., et al., “A model for the mechanism of human topoisomerase l,”Science, vol. 279, p. 1534-1541 (1998).
Wall M., et al., “The isolation and structure of camptothecin, a novel alkaloidal leukemia and tumor inhibitor from Camptotheca acuminata,”Journal of the American Chemical Society, vol. 88, p. 3888-3890 (1966).

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