Screening methods for biologically active ligands

Details Screening methods for biologically active ligands Screening methods for biologically active ligands

2001-07-17

2010-06-08

Landsman, Robert (Department: 1647)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C435S007200, C435S069700, C435S320100, C436S501000

Reexamination Certificate

active

07732133

ABSTRACT:
In the case when there are two objective biological activities, and the aim is to isolate a compound having at least one biological activity, the present inventors developed an assay method wherein a common detection marker is utilized for separately detecting the presence or absence of each of the biological activities. The present inventors discovered that a compound having at least one of two or more distinct biological activities can be efficiently and conveniently detected by simultaneously assaying at least one test sample or more by the above-mentioned method. Furthermore, for a test sample that proved to be positive by the detection method, they found that it is possible to efficiently and conveniently screen for a test sample having an objective specific biological activity by combining with a method wherein an individual activity of a test sample can be detected to specify the biological activity.

REFERENCES:
patent: 4859609 (1989-08-01), Dull et al.
patent: 5574136 (1996-11-01), Nagata et al.
patent: 62-272990 (1987-11-01), None
patent: 6-510531 (1994-11-01), None
patent: 8-508889 (1996-09-01), None
patent: WO 93/04373 (1993-03-01), None
patent: WO 94/29458 (1994-12-01), None
patent: WO9641169 (1996-12-01), None
patent: WO 01/25797 (2000-09-01), None
Friedrich et al., A two-step selection approach for the identification of ligand-binding determinants in cytokine receptors, 1998, Analytical Biochemistry, vol. 268, pp. 179-186.
Takahashi et al., Swapping between Fas and granulocyte colony-stimulating factor receptor, 1996, Journal Biological Chemistry, vol. 271, Issue 29, pp. 17555-17560.
Delrieu et al., IL-6 promoter is modulated by the 24 kDa FGF-2 isoform fused to the hormone binding domain of the oestrogen receptor, Cytokine, vol. 12, Issue 7, pp. 1110-1114.
Bork et al., Go hunting in sequence databases but watch out for the traps, 1996, Trends in Genetics, vol. 12, pp. 425-427.
Bork, Powers and Pitfalls in Sequence Analysis: the 70% hurdle, 2000, Genome Research, vol. 10, pp. 398-400.
Brenner, Errors in genome annotation, 1999, Trends in Genetics, vol. 15, pp. 132-132.
Doerks et al., Protein annotation: detective work for function prediction, 1998, Trends in Genetics, vol. 14, pp. 248-250.
Ngo et al., The protein folding problem and tertiary structure prediction,1994, pp. 492-495.
Skolnick et al., From genes to protein structure and function: novel applications of computational approaches in the genomic era, 2000, Trends in Biotech, vol. 18, Issue 1, pp. 34-39.
Smith et al., The challenge of genome sequence annotation or “the devil is in the details”, 1997, Nature Biotechnology, vol. 15, pp. 1222-1223.
Wells et al., Addivity of mutational effects in proteins, 1990, Biochemistry, vol. 29, pp. 8509-8517.
Geiger et al., “Integrated src kinase and costimulatory activity enhances signal transduction through single-chain chimeric receptors in T lymphocytes,” Blood, 98(8):2364-2371 (2001).
Seldin et al., “The Human IL4 Receptor Shares a Critical Signal Transduction Domain with Other Cytokine Receptors” J. Cell. Biochem. Suppl., Keystone Symposium on Hematopoiesis, Abstract No. M328, p. 89 (1992).
Ishizaka-Ikeda et al., “Signal transduction mediated by growth hormone receptor and its chimeric molecules with the granulocyte colony-stimulating factor receptor”,Proc. Natl. Aca. Sci. USA. vol. 90, pp. 123-127 (1993).
Kammer et al., “Homodimerization of interleukin-4 receptor alpha chain can induce intracellular signaling.” J. Biol. Chem. 271(39):23634 (1996).
Aritomi et al., “Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme,” Nature, 401(6754):713-717 (1999).
Boehm et al., “Rac-dependent anti-apoptotic signaling by the insulin receptor cytoplasmic domain,” J. Biol. Chem,. 274(40):28632-28636 (1999).
Boileau and Czajkowski, “Identification of transduction elements for benzodiazepine modulation of the GABA(A) receptor: three residues are required for allosteric coupling,” J. Neurosci., 19(23):10213-10220 (1999).
Jorrissen et al., “Characterization of a comparative model of the extracellular domain of the epidermal growth factor receptor,” Protein Sci., 9(2):310-324 (2000).
Koay and Sartorelli, “Functional differentiation signals mediated by distinct regions of the cytoplasmic domain of the granulocyte colony-stimulating factor receptor,” Blood, 93(11):3774-84 (1999).
Mikhailenko et al., “Functional domains of the very low density lipoprotein receptor: molecular analysis of ligand binding and acid-dependent ligand dissociation mechanisms,” J. Cell. Sci., 112 (Pt 19):3269-3281 (1999).
Muthukumaran et al., “Chimeric erythropoietin-interferon gamma receptors reveal differences in functional architecture of intracellular domains for signal transduction,” J .Biol. Chem., 272(8):4993-9 (1997).
Pelczar et al., “A conditional version of the Ets transcription factor Erm by fusion to the ligand binding domain of the oestrogen receptor,” Biochem. Biophys. Res. Commun., 239(1):252-256 (1997).
Prigent and Gullick, “Identification of c-erbB-3 binding sites for phosphatidylinositol 3′-kinase and SHC using an EGF receptor/c-erbB-3 chimera,” EMBO J., 13(12):2831-41 (1994).
Rogerson et al., “Structural determinants of aldosterone binding selectivity in the mineralocorticoid receptor,” J. Biol. Chem., 274(51):36305-11 (1999).
Scheer et al., “Structure-function relationships of the alpha1b-adrenergic receptor,” Eur. Urol., 36 (Suppl 1):11-16 (1999).
Ueno et al., “An epidermal growth factor receptor-leukocyte tyrosine kinase chimeric receptor generates ligand-dependent growth signals through the Ras signaling pathway,” J. Biol. Chem., 270(34):20135-42 (1995).
Villmann et al., “Kainate binding proteins possess functional ion channel domains,” J. Neurosci., 17(20):7634-43 (1997).
Yeagle et al., “Structure determination of the fourth cytoplasmic loop and carboxyl terminal domain of bovine rhodopsin,” Mol. Vis., 2:12 (1996).

Affiliated with

Also associated with

Rating

Screening methods for biologically active ligands does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Screening methods for biologically active ligands, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Screening methods for biologically active ligands will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4205458