Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1995-03-21
1996-12-17
Scheiner, Toni R.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
436501, 436536, 435 4, 435 792, 435 23, 435 24, G01N 3353
Patent
active
055852777
ABSTRACT:
A novel method for screening chemical compounds, test ligands, for potential pharmaceutical effectiveness. The disclosed method identifies possible therapeutic test ligands by placing them in the presence of target proteins and determining the ability of test ligands to increase the ratio of folded target protein to unfolded target protein. This differs significantly from known methods of novel pharmaceutical testing in that the biochemical function of the target protein need not be known and, except for one of the five embodiments of the method, the existence of any known ligands of the target protein is unnecessary.
REFERENCES:
Randall, L. K., Science, vol. 257, pp. 241-245 Jul. 10, 1992.
Grant, S. K., Biochemistry, vol. 31, pp. 9491-9501, 1992.
Tanigaki, N. et al., Human Immunology, vol. 36, pp. 119-127, 1993.
Zahnley, J. C., "Effects of Manganese and Calcium on Conformational Stability of Concanavalin A: A Differential Scanning Calorimetric Study", J. Inorganic Biochem. 15:67-78 (1981).
Grimaldi, S. et al., "Effects of Thyroxine Binding on the Stability, Conformation, and Fluorescence Properties of Thyroxine-Binding Globulin", Biochem. 21:145-151 (1982).
Pace, C. N. and T. McGrath, "Substrate Stabilization of Lysozyme to Thermal and Guanidine Hydrochloride Denaturation", J. Biol. Chem. 255:3862-3865 (1980).
Doyle, R. J. et al., "Stabilization of Concanavalin A by Metal Ligands", Carbohydrate Res. 46:111-118 (1976).
Casas-Finet, J. R. et al., "Mammalian Heterogeneous Ribonucleoprotein A1 and Its Constituent Domains", J. Mol. Biol. 229:873-889 (1993).
Merabet, E. K. et al., "Differential Scanning Calorimetric Study of 5-Enolpyruvoyl Shikimate-3-Phosphate Synthesis and its Complexes with Shikimate-3-Phosphate and Glyphosate . . . ", Biochim. Biophys. Acta 1161:272-278 (1993).
Fernandez-Ballester, G. et al., "Protein Stability and Interaction of the Nicotinic Acetylcholine Receptor With Cholinergic Ligands Studied by Fourier-Transform Infrared Spectroscopy", Biochem. J. 288:421-426 (1992).
Harrington, J. P., "Spectroscopic Analysis of the Unfolding of Transition Metal-Ion Complexes of Human Lactoferrin and Transferrin", Int. J. Biochem. 24:275-280 (1992).
Green, S. M. et al., "Roles of Metal Ions in the Maintenance of the Tertiary and Quaternary Structure of Arginase from Saccharomyces cerevisiae", J. Biol. Chem. 266:21474-21481.
Feng, Y. and S. G. Sligar, "Effects of Heme Binding on the Structure and Stability of Escherichia coli Apocytochrome b.sub.562 ", Biochem. 30:10150-10155 (1991).
Parsell, D. A. and R. T. Sauer, "The Structural Stability of a Protein Is an Important Determinant of Its Proteolytic Susceptibility in Escherichia coli", J. Biol. Chem. 264:7590-7595 (1989).
Wagenhofer, M. et al., "Thermal Denaturation of Engineered Tet Repressor Proteins and Their Complexes with tet Operator and Tetracycline Studied by Temperature Gradient Gel electrophoresis", Anal. Biochem. 175:422-432 (1988).
Lapadat, M. A. and L. L. Spremulli, "Effect of Guanine Nucleotides on the Conformation and Stability of Chloroplast Elongation Factor Tu", J. Biol. Chem. 264:5510-5514 (1989).
Kuwajima, K. et al., "Influence of Ca.sup.2+ Binding on the Structure and Stability of Bovine .alpha.-lactalbumin Studies by Circular Dichroism and Nuclear Magnetic Resonance Spectra", Int. J. Peptide Protein Res. 27:18-27 (1986).
Rosengart et al, "Heparin Protects Heparin-Binding Growth Factor-I From Proteolytic Inactivation In Vitro", Biochem. Biophys. Res. Commun., 152(1):432-440 (Apr. 15, 1988).
Nilsson et al. "Proper and Improper Folding of Proteins in the Cellular Environment", Annu. Rev. Mirobiol., 45:607-635 (1991).
Friguet et al. "Immunochemical Analysis of protein confirmation", pp. 287-310 in Protein Structure A Practical Approach, Creighton, ed., IRL Press (Oxford), 1989.
Bowie James U.
Pakula Andrew A.
Scheiner Toni R.
Scriptgen Pharmaceuticals Inc.
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