Screening method for identifying ligands for target proteins

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals

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436501, 435 23, 435 24, 435 4, 435 792, G01N 3353

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active

056795823

ABSTRACT:
A novel method for screening chemical compounds, test ligands, for potential pharmaceutical effectiveness. The disclosed method identifies possible therapeutic test ligands by placing them in the presence of target proteins and determining the ability of test ligands to increase the ratio of folded target protein to unfolded target protein. This differs significantly from known methods of novel pharmaceutical testing in that the biochemical function of the target protein need not be known and, except for one of the five embodiments of the method, the existence of any known ligands of the target protein is unnecessary.

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Grant, S.K., et al, Biochemistry, vol. 31, pp. 9491-9501, 1992.
Randall, L.L., Science, vol. 257, pp. 241-245, Jul. 1992.

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