Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...
Reexamination Certificate
2007-05-08
2007-05-08
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
C435S004000, C435S015000
Reexamination Certificate
active
10777008
ABSTRACT:
A method of screening a substance of interest for heme independent modulation of enzymatic activity of soluble guanylyl cyclase (sGC) is disclosed, comprising (a) obtaining αβCys105mutant sGC enzyme; (b) determining activity of the mutant enzyme for forming cGMP from GTP in the presence of the substance of interest in a reaction medium; (c) determining activity as in step (b), except in the absence of the substance of interest; optionally, (d) including an activator other than the substance of interest in steps b) and c); e) comparing results of (b)–(d) to yield a comparison result; and f) from that value of that result, assessing activity of the substance of interest for modulating cGMP production by the mutant enzyme. Increased or decreased formation of cGMP in the presence of the substance of interest indicates activity of the substance for modulating heme independent cGMP production.
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PCT/US04/03853, PCT International Search Report dated Oct. 28, 2004.
Martin Emil
Murad Ferid
Board of Regents of the University of Texas System
Conley & Rose, P.C.
Gitomer Ralph
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