Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-08-26
2000-05-02
Fredman, Jeffrey
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 4351723, 536231, 536 243, 536 2431, 536 2432, 536 2433, 536 254, 436501, C12Q 168, C12P 1934, C07H 2102, C07H 2104
Patent
active
060571039
ABSTRACT:
Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nucleic acid directly isolated from the environment; and (ii) screening said libraries utilizing an assay system. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.
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Dynal Technical Handbook, "Biomagnetic Techniques in Molecular Biology", pp. 78-89, 1995.
Nakamura et al, "The murine lymphotoxin-B receptor cDNA: isolation by the signal sequences trap and chromosomal mapping", Genomics 30:312-319, 1995.
Eberwine et al, "Isolation of enzyme cDNA clones by enzyme immunodetection assay: Isolation of a peptide acetyltransferase", Proc. Natl. Acad. Sci. 84:1449-1453, Mar. 1987.
Diversa Corporation
Fredman Jeffrey
Haile Lisa A.
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