Screening for novel bioactivities

Details Screening for novel bioactivities Screening for novel bioactivities

1997-10-06

2000-02-29

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1934

Patent

active

06030779&

ABSTRACT:
Disclosed is a process for identifying clones having a specified enzyme activity by screening for the specified enzyme activity in a library of clones prepared by (i) selectively isolating target nucleic acid from nucleic acid derived from at least one microorganism, by use of at least one polynucleotide probe comprising at least a portion of a nucleic acid sequence encoding an enzyme having the specified enzyme activity; and (ii) transforming a host with isolated target nucleic acid to produce a library of clones which are screened for the specified enzyme activity.

REFERENCES:
patent: 5783431 (1998-07-01), Peterson et al.
Clontech Catalogue pp. 75,91, 1995.
Maniatis et al. Molecular Cloning, pp. 224-240, 270-274, 1982.
Osuna et al., Combinatorial mutagenesis of three major groove-contacting residues of EcoRI: single and double amino acid replacements retaining methyltransferase-sensitive activity, Gene. 1991, vol. 106, pp. 7-12.
Dube et al., Artificial mutants generated by the insertion of random oligonucleotides into the putative nucleoside binding site of the HSV-1 thymidine kinase gene, Biochemistry, 1991, vol. 30, pp. 11760-11767.
Burioni et al., Engineering human monoclonal antibody fragments: A recombinant enzyme-linked Fab. Microbiologica., Apr. 1995, vol. 18, pp. 127-133.
Borrego et al., Combinatorial libraries by cassette mutagenesis, Nucleic Acid Research, 1995, vol. 23, No. 10, pp. 1834-1835.

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