Screening assays for cancer chemopreventative agents

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435 4, 435 721, 435 723, 435 19, 435 21, 436 63, C12Q 134, C12Q 100, C12Q 142

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058742352

ABSTRACT:
Nonsteroidal anti-inflammatory drugs cause a dramatic increase in intracellular ceramide, which induces apoptosis. The ceramide increase is likely mediated by cyclooxygenase inhibition, which elevates arachidonic acid, which stimulates sphingomyelinase, which produces ceramide. Contacting members of this pathway with test compounds and observing their effects provides a method of screening for potential cancer chemopreventative agents.

REFERENCES:
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