Screening assays for agonists and antagonists of the...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007100, C435S007200, C435S069100, C435S325000, C435S348000, C435S320100, C435S252300, C435S254200, C536S023100, C536S023500, C530S350000

Reexamination Certificate

active

07060450

ABSTRACT:
The present invention concerns the discovery that proteins encoded by a family of vertebrate genes, termed here hedgehog-related genes, comprise morphogenic signals produced by embryonic patterning centers, and are involved in the formation of ordered spatial arrangements of differentiated tissues in vertebrates. The present invention makes available compositions and methods that can be utilized, for example to generate and/or maintain an array of different vertebrate tissue both in vitro and in vivo.

REFERENCES:
patent: 5223408 (1993-06-01), Goddel et al.
patent: 5585087 (1996-12-01), Lustig et al.
patent: 5837538 (1998-11-01), Scott et al.
patent: 6027882 (2000-02-01), Scott et al.
patent: WO 90/02809 (1990-03-01), None
patent: WO 92/15679 (1992-09-01), None
Niswander, L., S. Jeffrey. G.R. Martin, and C. Tickle. A positive feedback loop coordinates growth and patterning in the vertebrate limb. Oct. 13, 1994. Nature. vol. 371. pp. 609-612.
Forbes, A.J., Y. Nakano, A.M. Taylor, and P.W.Ingham. Genetic analysis of hedgehog signalling inDrosophila embryo. 1993. Development Supplement. 115-124.
Bowie et al., “Deciphering the Message in the Protein Sequences: Tolerance to Amino Acid Substitutions”, Science 247:1306-1310. 1990.
Creighton, T.E. Proteins Structures and Molecular Principles, W.H. Freeman and Company: New York, N.Y. pp. 223-227. 1984.
Dingermanse, M.A. et al., “The expression of liver-specific genes with rat embryonic hepatocytes in a discontinuous process”, Differentiation 56: 153-162. 1994.
Lerner, R.A., Antibodies of predetermined specificity in biology and medicine, Adv. Immunol., 36: 1-44. 1984.
Zardoya et al., “Evolution and orthology of hedgehog genes”, Trends in Genetics. 12(12): 496-497. 1996.
Anderson, R. et al., “Maintenance of ZPA signaling in cultured mouse limb bud cells”,Devel. 117:142-1433 (1993).
Angier, N. “Biologists find key genes that shape patterning of embryos”,New York Times, Jan. 11, 1994, C-1.
Basler, KI., and G. Struhl, “Compartment boundaries and the control ofDrosophilalimb pattern by hedgehog protein”,Nature 368:208--214 (1994).
Basler, K. et al., “Control of Cell Pattern in the Neural Tube: Regulation of Cell Differentiation by dorsalin-1, a Novel TGFβ Family Member”, Cell 73: 687-702 (May 21, 1993).
Bass, S. et al., “Hormone phage: An Enrichment Method for Variant Proteins with Altered Binding Properties”, Proteins:Structure, Function, and Genetics 8:309-314 (1990).
Bejsovec, A. and E. Wieschaus, “Segment polarity gene interactoins modulate epidermal patterning inDrosophilaembryos”,Devel. 119:501-517 (1993).
Bienz, M., “Homeotic genes and positional signalling in theDrosophila viscera”,TIG 10:22-26 (Jan. 1994).
Bitgood, M. and McMahon, A., “Hedgehod and Bmp Genes are Coexpressed at Many Diverse Sites of Cell-Cell Internation in the Mouse Embryo”,Dev. Biol. 172(1):126-138 (1995).
Blair, S.S., “Hedgehog digs up an old Friend”,Nature 373:656-657 (Feb. 23, 1995).
Brand-Saberi, B. et al., “The ventralizing effect of the notochord on somite differentiation in chick embryos”,Anat. Embryol. 188:239-245 (1993).
Brockes, J., We may not have a morphogen,Nature 350:15 (1991).
Bumcrot, D.A. and McMahon A. “Sonic Hedgehog: Making the gradient”,Chemistry and Biology 3(1):13-16 (Jan. 1996).
Bumcrot, D.A. and McMahon, A., “Sonic signals somites”,Curr. Biol. 5(6):612-614 (Jun. 1995).
Bumcrot, D.A. et al., “Proteolytic Processing yields two secreted forms on sonic Hedgehog”,Mol. Cell. Biol. 15(4):2294-2302 (Apr. 1995).
Chang et al., Products, genetic linkage and limb pattening activity of a murine hedgehog gene, Development 120:3339-3353, Nov. 1994.
Charité, J. et al., “Ectopic Expression of Hoxb-8 Causes Duplication of the ZPA in the Forelimb and Homeotic Transformation of Axial Structures”,Cell 78:589-601 (1994).
Coffman et al., “Xotch, the Xenopus homolog ofDrosophilanotch ”,Science 249:1438-1441 (1990).
Concordet, J. and Ingham, P., “Developmental biology. Patterning goes sonic”,Nature 375(6529):279-280 (May 1995).
Currie et al., “Induction of a specific muscle cell type by a hedgehod-like protein in zebrafish”,Nature 383:452-455 (1996).
Curry et al., “Sequence analysis reveals homology between two proteins of the flagellar radial spoke”,Mol. Cell. Biol. 12:3967-3977 (1992).
Davidson, E.H., “How embryos work: a comparative view of diverse modes of cell fate specification”,Devel. 108:365-389 (1990).
Davis, A.P. and M.R. Capecchi, “Axial homeosis and appendicular skeleton defects in mice with a targeted disruption of hoxd-1”,Devel. 120:2187-2198 (1994).
Dickinson W., “Molecules and morphology: Where's the homology”,TIG 11, (4):119-120 (1995).
Dollé, P. et al., “Coordinate expression of the murine Hox-5 complex homoeobox-containing genes during limb pattern formation”,Nature 342:767-772 (1989).
Echelard, Y, et al., “Sonic Hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity”,Cell 75:1417-1430 (1993).
Ekker, S. et al., “Distinct expression and shared activities of members of the hedgehog gene family ofXenopus laevis”, Devel. 121(8):2337-2347 (Aug. 1995).
Ericson, J. Et al., “Sonic hedgehog induces the differentiation of ventral forebrain neurons: a common signal for ventral patterning within the neural tube”,Cell 81(5):747-756 (Jun. 1995).
Ettelaie, C. et al., “The effect of lipid peroxidation and lipolysis on the ability of lipoproteins to influence thromboplastin activity”,Biochim. Biophys. Acta. 1257(1):25-30 (Jun. 1995).
Fahmer, K. et al., “Transcription of H-2and Qa genes in embryonic and adult mice”,EMBO J. 6:1265-1271 (1987).
Fallon, J.F. et al., “FGF-2: Apical ectodermal ridge growth signal for chick limb development”,Science 264:104-107(1994).
Fan, C. et al., “Long-range sclerotome induction by sonic hedgehod: Direct role of the amino-terminal cleavage product and modulation by the cyclic AMP signaling pathway”,Cell 81: 457-465 (May 5, 1995).
Fietz, M. et al., The hedgehog gene family inDrosophilaand vertebrate development,Develop. Supp: 43-51 (1994).
Forbes, A.J., et al., “Genetic analysis of hedgehog signalling in theDrosophilaembryo”,Devel. 119(Supp.): 115-124 (1993).
Francis, P.H. et al., “Bone morphogenetic proteins and a signalling pathway that controls patterning in the developing chick limb”,Devel. 120:209-218 (1994).
Gallop, J., et al., “Applications of combinatorial technologies to drug discovery. 1. Background and peptide combinatorial libraries”,J. of Mec. Chem. 37(9):1233-1251 (1994).
Gérard, M. et al., “Structure and activity of regulatory elements involved in the activation of the Hoxd-11 gene during late gastrulation”,EMBO J. 12:3539-3550 (1993).
Gurdon, J.B., “The Generation of diversity and pattern in animal development”,Cell 68:185-199 (1992).
Gustin, K. et al., “Characterization of the role of individual protein binding motifs within the hepatitis B virus enhancer 1 on X promoter activity using linker scanning mutagenies”,Virolgy 193:653-660 (1993).
Hall, T., et al., “A potential catalytic site revealed by the 1.7-A crystal structure of the amino-terminal signalling domain of Sonic hedgehog”,Nature 378(6553):212-216(Nov. 1995).
Halpern, M.E., et al., “Induction of muscle pioneers and floor plate is distinguished by the zebrasish no tail mutation”,Cell 75:99-111 (1993).
Hamburger, V. and H.L. Hamilton, “A series of normal stages in the development of the chick embryo”,J. Morph. 88:49-92 (1951).
Hammerschmidt, M. et al., “The world according to hedgehog”,TIG 13(1):14-21 (19

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