Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-11-15
1995-12-12
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 691, 435 704, 4352402, C12Q 168
Patent
active
054748976
ABSTRACT:
A method for identifying compounds capable of inducing immunosuppression by inhibiting the CD28 signal transduction pathway and T cell comprises exposing cultured T cells to one or more test compounds. The T cells are obtained from a T cell line which stably incorporates DNA sequence comprising in reading frame an enhancer region responsive to a CD28-regulated nuclear binding protein and a marker gene. The cells are cultured under conditions which result in activation of both the T cell receptor and the CD28 receptor, resulting in enhanced expression of the marker gene. Test compounds which inhibit expression of the marker gene are considered as candidates for immunosuppressive drugs.
REFERENCES:
patent: 4868112 (1989-09-01), Toole, Jr.
patent: 5053337 (1991-10-01), Weinshank et al.
patent: 5082774 (1992-01-01), Heinrich
Ohashi, P. S. et al. "Reconstruction of an active surface Te/T-cell antigen receptor by DNA transfer." Nature vol. 316, No. 6029 pp. 606-609, 15 Aug. 1985.
Saito T. et al., "Specific antigen-Ia activation of transfected human T cells expressing murine Ti .alpha..beta.-human T3 receptor complexes." Nature vol. 35, pp. 125-130, 8 Jan. 1987.
Maxwell, I. H. et al. "Electroporation of Mammalian Cells with a Firefly Luciferase Expression Plasmid" DNA vol. 7, No. 8, pp. 557-562, 1988.
Sczakiel, G. et al. "Testing for Electrotransfection Parameters by use of the Fluorescent Dye Lucifer yellow CH." Analytical Biochemistry No. 181, pp. 309-314, 1989.
Novak, T. J., et al. "Differential Transient and Long-Term Expression of DNA Sequences Introduced into T-Lymphocyte Lines" DNA vol. 5 No. 6, pp. 439-451, 1986.
Kay and Benzie, Immunol. Letters, 23, 155-160 (1989).
Sambrook et al., Molecular Cloning, Cold Spring Harbor Lab. Press, pp. 16.39-16.40, 1989.
Fraser James
Weiss Arthur
Horlick Kenneth R.
Jones W. Gary
The Regents of the University of California
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