Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-06-22
1994-04-26
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4351721, 4351723, 435 721, 435 723, 4352351, 436501, 536 231, 536 234, 536 235, 536 236, 536 237, C12Q 168, C12Q 100, G01N 33566, C07H 1700
Patent
active
053066197
ABSTRACT:
The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Also described herein is a method to capture DNA that has been released from the DNA:protein complex.
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Andrews Beth M.
Cantor Charles R.
Edwards Cynthia A.
Dehlinger Peter J.
Fabian Gary R.
Genelabs Technologies, Inc.
Hill Jr. Robert J.
Wang Gian P.
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