Screening antisense and ribozyme nucleic acids in schizosaccharo

Details Screening antisense and ribozyme nucleic acids in schizosaccharo Screening antisense and ribozyme nucleic acids in schizosaccharo

1996-10-18

1999-08-03

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 6, 4351723, 536 245, C12Q 102, C12Q 168, C07H 2104

Patent

active

059324350

DESCRIPTION:

BRIEF SUMMARY
The present invention relates generally to an in vivo system for gene expression and, more particularly, to the use of the system to screen for molecules which are capable of inhibiting, reducing, altering or otherwise modulating the expression of a target nucleotide sequence or the activity of a gene product. The in vivo system of the present invention is particularly but not exclusively useful for screening for antisense, sense or ribozyme constructs or transdominant polypeptides, small peptides or other chemical compounds and which are capable of inhibiting, reducing, altering or otherwise modulating expression of target genes or target genetic sequences or the activity of target gene products of commercial importance such as in the medical, agricultural and industrial fields.
Sequence Identity Numbers (SEQ ID NOs.) for the nucleotide sequences referred to in the specification are defined just prior to the claims.
Throughout this specification, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or integer or group of elements or integers but not the exclusion of any other element or integer or group of elements or integers.
The rapidly increasing sophistication of recombinant DNA technology is greatly facilitating the efficacy of many commercially important industries including areas of medicine, agriculture, horticulture and fermentation. An important tool in recombinant DNA technology is the use of antisense molecules, sense molecules, ribozymes and other genetic sequences and/or non-nucleotide molecules such as peptides or chemical agents to affect expression of genetic sequences.
Ribozymes are RNA molecules which possess highly specific endoribonuclease activity. In particular, they comprise a hybridising region which is complementary in nucleotide sequence to at least part of a target RNA and a catalytic region which is adapted to cleave the target RNA. An example where ribozymes are well described is Haseloff J. and Gerlach W Nature 334: 586-591, 1988 and in International Patent Application No. WO 89/05852. Antisense molecules are genetic constructs which are generally complementary in nucleotide sequences to target mRNA. Although the exact mode of action of antisense molecules is unclear, it is possible that they form a duplex with all or part of target mRNA with a consequential interferring effect on the mRNA transcript. Sense nucleotide constructs are used in co-suppression and have been shown to be effective in reducing expression of, for example, plant genes.
There is a need to develop effective approaches for rapidly evaluating molecules such as antisense, sense and ribozyme constructs, transdominant polypeptides, small peptides and other chemical compounds as potential effector molecules in modulating expression of target genetic sequences or the activity of products encoded thereby.
Microorganisms have previously been considered as a convenient in vivo model system for testing for compounds which affect, for example, their viability or ability to grow. Microorganisms are advantageous experimental hosts for molecular and genetic analysis. Their assets include a short generation time, readily available techniques for growing and analysing large numbers of cells and the relative ease of introducing and recovering nucleic acids from these cells. More than 10.sup.11 microorganisms can readily be grown on inexpensive nutrients whereas the production of even 10.sup.8 mammalian cells is slow and expensive. However, while bacterial cells have been used in the elucidation of many basic aspects of gene expression, they lack many of the fundamental features of eukaryotic RNA physiology. Bacterial cells have no nuclear compartment, contain a reduced number of completely distinct RNA and DNA binding proteins, have few or no spliced mRNAs, no spliceosomal machinery for intron removal, and use a different system for the initiation of protein translation.
Yeast cells, on the other hand,

REFERENCES:
patent: 5443962 (1995-08-01), Draetta et al.
Forsburg, "Comparison of Schizosaccharomyces pombe expression systems", Nucleic Acids Res. 21(12): 2955-2956, Jun. 1993.
Fleig et al., "A dominant negative allele of p34-cdc2 shows altered phosphoamino acid content and sequesters p56-cdc13 cyclin", Mol. Cell. Biol. 12(3): 2295-2301, May 1992.
Schweingruber et al., "Regulation of pho1-encoded acid phophatase of Schizzosaccharomyces pombe by adenine and phosphate", Curr. Genet. 22: 289-292, Oct. 1992.
Atkins et al., "Artificial ribozyme and antisense gene expression in Saccharomyces cerevisiae", Antisense Res. Devel. 4: 109-117, 1994.

Affiliated with

Also associated with

Rating

Screening antisense and ribozyme nucleic acids in schizosaccharo does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Screening antisense and ribozyme nucleic acids in schizosaccharo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Screening antisense and ribozyme nucleic acids in schizosaccharo will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-848729