Screening and characterization of glutaryl-7-aminocephalosporani

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435 74, 435 712, 435228, C12Q 134, G01N 3352, C12P 2100, C12N 950

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active

057668710

ABSTRACT:
A method of screening for a given enzyme in colony-forming cells using a substrate of the enzyme that has low solubility. Use of such a method leads to the discovery of glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase of Pseudomonas nitroreducens. Also disclosed is a method of obtaining GL-7-ACA acylase from Pseudomonas cells.

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