Screen for CDC7 inhibitors

Details Screen for CDC7 inhibitors Screen for CDC7 inhibitors

2005-10-11

2005-10-11

Guzo, David (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

C435S006120, C435S254100, C435S254110, C435S255100, C435S255700, C435S471000

Reexamination Certificate

active

06953672

ABSTRACT:
Disclosed is a yeast cell whose genetic complement includes an inactive allele of the yeast CDC7 gene, a first nucleic acid that encodes a mammalian Cdc7 protein, and a second nucleic acid that encodes a mammalian Dbf4 protein. The yeast cell is dependent on the mammalian Cdc7 and Dbf4 proteins for viability. The yeast cell can be used to identify potential anti-proliferative agents by virtue of their inhibition of the mammalian Cdc7 and Dbf4 proteins. In some embodiments, a control yeast cell which does not depend on Cdc7 for viability is used in a secondary screen.

REFERENCES:
Jiang, W. et al. “Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication”, 1999, EMBO journal, vol. 18. pp. 5703-5713.
Johnston, LH et al. “First the CDKs, now the DDKs”, 1999, Trends in Cell Biology, vol. 9, p249-252.
Kim, JM et al. “Growth Regulation of the Expression of Mouse cDNA and Gene Encoding a Serine/Threonine Kinase related to S. Cerevisiae CDC7 Essential for G1/S Transition”, 1998, J. Biol. Chem. vol. 273, p. 23248-23257.

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