Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1993-07-22
1996-02-27
Walsh, Stephen G.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
530324, 4242781, 930260, 435 691, A61K 3817, C07K 14435
Patent
active
054948959
ABSTRACT:
A thirty-nine amino acid peptide, Margatoxin (MgTX), is purified to homogeneity from venom of the scorpion Centruroides margaritatus. The gene encoding MgTX is constructed and this gene is expressed in E. coli, to produce recombinant MgTX. MgTX is a potent and selective inhibitor of a voltage-dependent K.sup.+ channel present in human lymphocytes. MgTX exhibits immunosuppressant activity with human T-lymphocytes, and is useful as an immunosuppressant, in modeling nonpeptidyl K.sup.+ channel blockers, and in establishing biochemical assays based on ligand binding or other protocols with which to screen for other novel modulators of voltage dependent K.sup.+ channels in lymphocytes and other tissues including the brain. As an immunosuppressant, MgTX is useful in the treatment of autoimmune diseases, the prevention of rejection of foreign organ transplants and/or related afflictions, diseases and illnesses.
REFERENCES:
patent: 4929718 (1990-05-01), Possani et al.
patent: 4960867 (1990-10-01), Garcia et al.
patent: 5006512 (1991-04-01), Ohnishi
Park et al, PNAS 88:2046-2050 (Mar. 1991).
Park et al, "Design, synthesis, and functional expression of a gene for charybdotoxin . . . " PNAS 88(6):2046-2050 (1991); abstract #118447 in Biol. Abst. 91(11):AB-458 (Jun. 1991).
Possani et al "The Primary Structure of Noxiustoxin . . . " Carlsberg Res. Commun. 47: 285-289 (1982).
R. J. Leonard et al., "Selective Blockers of Voltage-gated K channels depolarize human T lymphocytes Mechanism of the antiproliferative effect of charybdotoxin," Proc. Natl Acad. Sci. vol. 89:, pp. 10094-10098 (Nov. 1992).
C. S. Lin et al., "Voltage-gated Potassium Channels Regulate Calcium-dependent Pathways Involved In Human T-Lymphocyte Activation" J. Exp. Med., vol. 177: pp. 637-644, (Mar. 1993).
K. G. Chandy et al., "Voltage-Gated Potassium Channels Are Required For Human T Lymphocyte Activation", J. Exp. Med., vol. 160:, pp. 369-385 (1984).
DeCoursey et al., "Voltage-gated K Channels In Human T Lymn Mitogenesis?", Nature, vol. 307., pp. 465-468 (1984).
J. Novick et al., "Purification & Characterization of Two Novel Peptidyl Toxins Directed against K Channels from Venom of New World Scorpions" Poster Presentation, Feb. 25, 1991.
M. Price et al., "Charybdotoxin Inhibits Proliferation & Interleukin 2 Production in Human Peripheral Blood Lymphocytes", Proc. Natl. Acad. Sci. USA, vol. 86:, pp. 10171-10175 (Dec. 1989).
J. Vazquez, "Characterization of High Affinity Binding Sites for Charybdotoxin in Synaptic Plasma Membranes from Rat Brains", J. Biol Chem, vol. 256:, pp. 15564-15571 (1990).
M. Garcia-Calvo, "Purification, Characterication, & Biosynthesis of Margatoxin, A Component of Centruroides Margaritatus Venom That Selectively Inhibits Voltage-dependent Potassium Channels", J. Biol. Chem., vol. 268:, pp. 118866-18874 (Jul. 1993).
J. Novick et al., "Purification & Characterization of 2 Novel Peptidyl Toxins directed Against K Channels From Venom of New World Scorpions" Biophys J, vol. 59, p. 78a (1991) (Abstr.) #124.
R. Leonard et al., "K Channels In Human T-Cells (HTC); Regulation of Membrane Potential." Biophys. J. vol. 61: Abstract, p. A380. (1992) (Abstr.) #2187.
R. S. Slaughter et al., "Inhibition by Toxins of Charybdotoxin Binding To The Voltage-Gated Potassium Channel of Lymphocytes: Correlation with Block of Activation of Human Peripheral T-Lymphocytes." Biophys J., vol. 59:, p. 213a (1991) (abstr.) #TuPM-A2.
Garcia Maria L.
Koo Gloria C.
Leonard Reid J.
Lin Chiu-Chuan S.
Slaughter Robert S.
Bigley Francis P.
Daniel Mark R.
Merck & Co. , Inc.
Walsh Stephen G.
LandOfFree
Scorpion peptide margatoxin with immunosuppressant activity does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Scorpion peptide margatoxin with immunosuppressant activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Scorpion peptide margatoxin with immunosuppressant activity will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1679340